Inactivation of CMP-N-acetylneuraminic acid hydroxylase occurred prior to brain expansion during human evolution

Hsun Hua Chou, Toshiyuki Hayakawa, Sandra Diaz, Matthias Krings, Etty Indriati, Meave Leakey, Svante Paabo, Yoko Satta, Naoyuki Takahata, Ajit Varki

Research output: Contribution to journalArticle

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Abstract

Humans are genetically deficient in the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) because of an Alu-mediated inactivating mutation of the gene encoding the enzyme CMP-N-acetylneuraminic acid (CMP-Neu5Ac) hydroxylase (CMAH). This mutation occurred after our last common ancestor with bonobos and chimpanzees, and before the origin of present-day humans. Here, we take multiple approaches to estimate the timing of this mutation in relationship to human evolutionary history. First, we have developed a method to extract and identify sialic acids from bones and bony fossils. Two Neandertal fossils studied had clearly detectable Neu5Ac but no Neu5Gc, indicating that the CMAH mutation predated the common ancestor of humans and Neandertals, ≈0.5-0.6 million years ago (mya). Second, we date the insertion event of the inactivating human-specific sahAluY element that replaced the ancestral AluSq element found adjacent to exon 6 of the CMAH gene in the chimpanzee genome. Assuming Alu source genes based on a phylogenetic tree of human-specific Alu elements, we estimate the sahAluY insertion time at ≈2.7 mya. Third, we apply molecular clock analysis to chimpanzee and other great ape CMAH genes and the corresponding human pseudogene to estimate an inactivation time of ≈2.8 mya. Taken together, these studies indicate that the CMAH gene was inactivated shortly before the time when brain expansion began in humankind's ancestry, ≈2.1-2.2 mya. In this regard, it is of interest that although Neu5Gc is the major sialic acid in most organs of the chimpanzee, its expression is selectively down-regulated in the brain, for as yet unknown reasons.

Original languageEnglish
Pages (from-to)11736-11741
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number18
DOIs
Publication statusPublished - Sep 3 2002

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Pan troglodytes
Brain
N-Acetylneuraminic Acid
Neanderthals
Mutation
Fossils
Genes
Pan paniscus
Alu Elements
Sialic Acids
Cytidine Monophosphate
Pseudogenes
Hominidae
CMPacetylneuraminate monooxygenase
Exons
History
Genome
Bone and Bones
Enzymes

All Science Journal Classification (ASJC) codes

  • General

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Inactivation of CMP-N-acetylneuraminic acid hydroxylase occurred prior to brain expansion during human evolution. / Chou, Hsun Hua; Hayakawa, Toshiyuki; Diaz, Sandra; Krings, Matthias; Indriati, Etty; Leakey, Meave; Paabo, Svante; Satta, Yoko; Takahata, Naoyuki; Varki, Ajit.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 18, 03.09.2002, p. 11736-11741.

Research output: Contribution to journalArticle

Chou, Hsun Hua ; Hayakawa, Toshiyuki ; Diaz, Sandra ; Krings, Matthias ; Indriati, Etty ; Leakey, Meave ; Paabo, Svante ; Satta, Yoko ; Takahata, Naoyuki ; Varki, Ajit. / Inactivation of CMP-N-acetylneuraminic acid hydroxylase occurred prior to brain expansion during human evolution. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 18. pp. 11736-11741.
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abstract = "Humans are genetically deficient in the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) because of an Alu-mediated inactivating mutation of the gene encoding the enzyme CMP-N-acetylneuraminic acid (CMP-Neu5Ac) hydroxylase (CMAH). This mutation occurred after our last common ancestor with bonobos and chimpanzees, and before the origin of present-day humans. Here, we take multiple approaches to estimate the timing of this mutation in relationship to human evolutionary history. First, we have developed a method to extract and identify sialic acids from bones and bony fossils. Two Neandertal fossils studied had clearly detectable Neu5Ac but no Neu5Gc, indicating that the CMAH mutation predated the common ancestor of humans and Neandertals, ≈0.5-0.6 million years ago (mya). Second, we date the insertion event of the inactivating human-specific sahAluY element that replaced the ancestral AluSq element found adjacent to exon 6 of the CMAH gene in the chimpanzee genome. Assuming Alu source genes based on a phylogenetic tree of human-specific Alu elements, we estimate the sahAluY insertion time at ≈2.7 mya. Third, we apply molecular clock analysis to chimpanzee and other great ape CMAH genes and the corresponding human pseudogene to estimate an inactivation time of ≈2.8 mya. Taken together, these studies indicate that the CMAH gene was inactivated shortly before the time when brain expansion began in humankind's ancestry, ≈2.1-2.2 mya. In this regard, it is of interest that although Neu5Gc is the major sialic acid in most organs of the chimpanzee, its expression is selectively down-regulated in the brain, for as yet unknown reasons.",
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AU - Leakey, Meave

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