Inactivation of Escherichia coli DnaA protein by DNA polymerase III and negative regulations for initiation of chromosomal replication

Tsutomu Katayama, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Genetic and biochemical evidence indicates that initiation of chromosomal replication in Escherichia coli occurs in a nucleoprotein complex at the replication origin (oriC) formed with DnaA protein. The frequency of initiation at oriC is tightly regulated to only once per chromosome per cell cycle. To prevent untimely, extra initiations, negative control for initiation is indispensable. Recently, we found that the function of the initiator protein, DnaA, is controlled by DNA polymerase III holoenzyme, the replicase of the chromosome. The ATP-bound form of DnaA protein, an active form for initiation, is efficiently converted to the ADP bound form, an inactive form, since a subunit of the polymerase loaded on DNA (β subunit sliding clamp) stimulates hydrolysis of ATP bound to DnaA protein. Comparison of this system, RIDA (regulatory inactivation of DnaA), with other systems for negative regulation of initiation is included in this review, and the roles of these systems for concerted control for initiation during the cell cycle are discussed.

Original languageEnglish
Pages (from-to)835-840
Number of pages6
JournalBiochimie
Volume81
Issue number8-9
DOIs
Publication statusPublished - Jan 1 1999

Fingerprint

DNA Polymerase III
Escherichia coli Proteins
Escherichia coli
Chromosomes
Cell Cycle
Proteins
Adenosine Triphosphate
Cells
Holoenzymes
Replication Origin
Nucleoproteins
Clamping devices
Adenosine Diphosphate
Molecular Biology
Hydrolysis
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Inactivation of Escherichia coli DnaA protein by DNA polymerase III and negative regulations for initiation of chromosomal replication. / Katayama, Tsutomu; Sekimizu, Kazuhisa.

In: Biochimie, Vol. 81, No. 8-9, 01.01.1999, p. 835-840.

Research output: Contribution to journalArticle

@article{a1524486baf2430da4aa8fd4bd2d4977,
title = "Inactivation of Escherichia coli DnaA protein by DNA polymerase III and negative regulations for initiation of chromosomal replication",
abstract = "Genetic and biochemical evidence indicates that initiation of chromosomal replication in Escherichia coli occurs in a nucleoprotein complex at the replication origin (oriC) formed with DnaA protein. The frequency of initiation at oriC is tightly regulated to only once per chromosome per cell cycle. To prevent untimely, extra initiations, negative control for initiation is indispensable. Recently, we found that the function of the initiator protein, DnaA, is controlled by DNA polymerase III holoenzyme, the replicase of the chromosome. The ATP-bound form of DnaA protein, an active form for initiation, is efficiently converted to the ADP bound form, an inactive form, since a subunit of the polymerase loaded on DNA (β subunit sliding clamp) stimulates hydrolysis of ATP bound to DnaA protein. Comparison of this system, RIDA (regulatory inactivation of DnaA), with other systems for negative regulation of initiation is included in this review, and the roles of these systems for concerted control for initiation during the cell cycle are discussed.",
author = "Tsutomu Katayama and Kazuhisa Sekimizu",
year = "1999",
month = "1",
day = "1",
doi = "10.1016/S0300-9084(99)00213-8",
language = "English",
volume = "81",
pages = "835--840",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier",
number = "8-9",

}

TY - JOUR

T1 - Inactivation of Escherichia coli DnaA protein by DNA polymerase III and negative regulations for initiation of chromosomal replication

AU - Katayama, Tsutomu

AU - Sekimizu, Kazuhisa

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Genetic and biochemical evidence indicates that initiation of chromosomal replication in Escherichia coli occurs in a nucleoprotein complex at the replication origin (oriC) formed with DnaA protein. The frequency of initiation at oriC is tightly regulated to only once per chromosome per cell cycle. To prevent untimely, extra initiations, negative control for initiation is indispensable. Recently, we found that the function of the initiator protein, DnaA, is controlled by DNA polymerase III holoenzyme, the replicase of the chromosome. The ATP-bound form of DnaA protein, an active form for initiation, is efficiently converted to the ADP bound form, an inactive form, since a subunit of the polymerase loaded on DNA (β subunit sliding clamp) stimulates hydrolysis of ATP bound to DnaA protein. Comparison of this system, RIDA (regulatory inactivation of DnaA), with other systems for negative regulation of initiation is included in this review, and the roles of these systems for concerted control for initiation during the cell cycle are discussed.

AB - Genetic and biochemical evidence indicates that initiation of chromosomal replication in Escherichia coli occurs in a nucleoprotein complex at the replication origin (oriC) formed with DnaA protein. The frequency of initiation at oriC is tightly regulated to only once per chromosome per cell cycle. To prevent untimely, extra initiations, negative control for initiation is indispensable. Recently, we found that the function of the initiator protein, DnaA, is controlled by DNA polymerase III holoenzyme, the replicase of the chromosome. The ATP-bound form of DnaA protein, an active form for initiation, is efficiently converted to the ADP bound form, an inactive form, since a subunit of the polymerase loaded on DNA (β subunit sliding clamp) stimulates hydrolysis of ATP bound to DnaA protein. Comparison of this system, RIDA (regulatory inactivation of DnaA), with other systems for negative regulation of initiation is included in this review, and the roles of these systems for concerted control for initiation during the cell cycle are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0032710590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032710590&partnerID=8YFLogxK

U2 - 10.1016/S0300-9084(99)00213-8

DO - 10.1016/S0300-9084(99)00213-8

M3 - Article

C2 - 10572296

AN - SCOPUS:0032710590

VL - 81

SP - 835

EP - 840

JO - Biochimie

JF - Biochimie

SN - 0300-9084

IS - 8-9

ER -