Inclusion of DNA into organic gelator fibers made of amphipathic molecules and its controlled release

Ryouji Karinaga, Yeonhwan Jeong, Seiji Shinkai, Kenji Kaneko, Kazuo Sakurai

Research output: Contribution to journalReview article

36 Citations (Scopus)

Abstract

When methyl 4,6-O-(p-nitrobenzylidene)-α-D-glucopyranoside (p-NO 2Glu) was dissolved in water, p-NO2Glu molecules self-assembled to form a fiber (elemental fiber), and as a result, the solution became a partially transparent gel. When an equal (or more) amount of DNA was added to the gel, a white and crystalline gel was obtained. Energy-dispersive X-ray spectroscopy coupled with TEM and confocal microscopy suggested that DNA was included in the gel fibers made of p-NO2Glu molecules. The results imply that p-NO2Glu molecules are self-assembled to form an elemental fiber and these elemental fibers and DNA are twisted together to form higher hierarchic fibers. When the complexed gel made of plasmid DNA (pDNA) and p-NO2Glu was added to E. coli T7 S30 extract solution, the pDNA had less expression ability compared with naked one. When we added methyl-β-cyclodextrin (MβCyD), the expression rate was recovered with increasing added amount of MβCyD. The present paper shows inclusion and controlled release of DNA from a novel supporting material of DNA and that technology could play an important role in the development of localized approaches to gene therapy.

Original languageEnglish
Pages (from-to)9398-9401
Number of pages4
JournalLangmuir
Volume21
Issue number21
DOIs
Publication statusPublished - Oct 11 2005

Fingerprint

DNA
deoxyribonucleic acid
inclusions
Molecules
fibers
Fibers
Gels
gels
molecules
plasmids
Cyclodextrins
Plasmids
gene therapy
Gene therapy
Confocal microscopy
Escherichia coli
Crystalline materials
Transmission electron microscopy
microscopy
transmission electron microscopy

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Inclusion of DNA into organic gelator fibers made of amphipathic molecules and its controlled release. / Karinaga, Ryouji; Jeong, Yeonhwan; Shinkai, Seiji; Kaneko, Kenji; Sakurai, Kazuo.

In: Langmuir, Vol. 21, No. 21, 11.10.2005, p. 9398-9401.

Research output: Contribution to journalReview article

Karinaga, Ryouji ; Jeong, Yeonhwan ; Shinkai, Seiji ; Kaneko, Kenji ; Sakurai, Kazuo. / Inclusion of DNA into organic gelator fibers made of amphipathic molecules and its controlled release. In: Langmuir. 2005 ; Vol. 21, No. 21. pp. 9398-9401.
@article{53e474b6bbc646d9a29140033cba6743,
title = "Inclusion of DNA into organic gelator fibers made of amphipathic molecules and its controlled release",
abstract = "When methyl 4,6-O-(p-nitrobenzylidene)-α-D-glucopyranoside (p-NO 2Glu) was dissolved in water, p-NO2Glu molecules self-assembled to form a fiber (elemental fiber), and as a result, the solution became a partially transparent gel. When an equal (or more) amount of DNA was added to the gel, a white and crystalline gel was obtained. Energy-dispersive X-ray spectroscopy coupled with TEM and confocal microscopy suggested that DNA was included in the gel fibers made of p-NO2Glu molecules. The results imply that p-NO2Glu molecules are self-assembled to form an elemental fiber and these elemental fibers and DNA are twisted together to form higher hierarchic fibers. When the complexed gel made of plasmid DNA (pDNA) and p-NO2Glu was added to E. coli T7 S30 extract solution, the pDNA had less expression ability compared with naked one. When we added methyl-β-cyclodextrin (MβCyD), the expression rate was recovered with increasing added amount of MβCyD. The present paper shows inclusion and controlled release of DNA from a novel supporting material of DNA and that technology could play an important role in the development of localized approaches to gene therapy.",
author = "Ryouji Karinaga and Yeonhwan Jeong and Seiji Shinkai and Kenji Kaneko and Kazuo Sakurai",
year = "2005",
month = "10",
day = "11",
doi = "10.1021/la0515524",
language = "English",
volume = "21",
pages = "9398--9401",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "21",

}

TY - JOUR

T1 - Inclusion of DNA into organic gelator fibers made of amphipathic molecules and its controlled release

AU - Karinaga, Ryouji

AU - Jeong, Yeonhwan

AU - Shinkai, Seiji

AU - Kaneko, Kenji

AU - Sakurai, Kazuo

PY - 2005/10/11

Y1 - 2005/10/11

N2 - When methyl 4,6-O-(p-nitrobenzylidene)-α-D-glucopyranoside (p-NO 2Glu) was dissolved in water, p-NO2Glu molecules self-assembled to form a fiber (elemental fiber), and as a result, the solution became a partially transparent gel. When an equal (or more) amount of DNA was added to the gel, a white and crystalline gel was obtained. Energy-dispersive X-ray spectroscopy coupled with TEM and confocal microscopy suggested that DNA was included in the gel fibers made of p-NO2Glu molecules. The results imply that p-NO2Glu molecules are self-assembled to form an elemental fiber and these elemental fibers and DNA are twisted together to form higher hierarchic fibers. When the complexed gel made of plasmid DNA (pDNA) and p-NO2Glu was added to E. coli T7 S30 extract solution, the pDNA had less expression ability compared with naked one. When we added methyl-β-cyclodextrin (MβCyD), the expression rate was recovered with increasing added amount of MβCyD. The present paper shows inclusion and controlled release of DNA from a novel supporting material of DNA and that technology could play an important role in the development of localized approaches to gene therapy.

AB - When methyl 4,6-O-(p-nitrobenzylidene)-α-D-glucopyranoside (p-NO 2Glu) was dissolved in water, p-NO2Glu molecules self-assembled to form a fiber (elemental fiber), and as a result, the solution became a partially transparent gel. When an equal (or more) amount of DNA was added to the gel, a white and crystalline gel was obtained. Energy-dispersive X-ray spectroscopy coupled with TEM and confocal microscopy suggested that DNA was included in the gel fibers made of p-NO2Glu molecules. The results imply that p-NO2Glu molecules are self-assembled to form an elemental fiber and these elemental fibers and DNA are twisted together to form higher hierarchic fibers. When the complexed gel made of plasmid DNA (pDNA) and p-NO2Glu was added to E. coli T7 S30 extract solution, the pDNA had less expression ability compared with naked one. When we added methyl-β-cyclodextrin (MβCyD), the expression rate was recovered with increasing added amount of MβCyD. The present paper shows inclusion and controlled release of DNA from a novel supporting material of DNA and that technology could play an important role in the development of localized approaches to gene therapy.

UR - http://www.scopus.com/inward/record.url?scp=27144523224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27144523224&partnerID=8YFLogxK

U2 - 10.1021/la0515524

DO - 10.1021/la0515524

M3 - Review article

C2 - 16207011

AN - SCOPUS:27144523224

VL - 21

SP - 9398

EP - 9401

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 21

ER -