INCOMPLETE REPAIR of RETINAL STRUCTURE after VITRECTOMY with INTERNAL LIMITING MEMBRANE PEELING

Toshio Hisatomi, Takashi Tachibana, Shoji Notomi, Shunji Nakatake, Kohta Fujiwara, yusuke murakami, Yasuhiro Ikeda, Shigeo Yoshida, Hiroshi Enaida, Toshinori Murata, Taiji Sakamoto, Kohei Sonoda, Tatsuro Ishibashi

Research output: Contribution to journalArticle

Abstract

Purpose: To examine retinal changes after vitrectomy with internal limiting membrane (ILM) peeling, we used a cynomolgus monkey model and focused on surgical damages of ILM peeling for long observational period of 3 years. Methods: Vitrectomy was performed followed by ILM peeling similar to clinical settings in humans. Ultrastructural changes of the retina were investigated by light, transmission, and scanning electron microscopy at 3 months and 3 years after ILM peeling. Results: Ultrastructural study showed that the ILM peeled area was still clearly recognized after 3 years. The Müller cell processes covered most of the retina; however, the nerve fiber layer was partly uncovered and exposed to the vitreous space. The arcuate linear nerve fiber bundles were observed as comparable with dissociated optic nerve fiber layer appearance. Small round retinal surface defects were also observed around macula, resembling the dimple sign. Forceps-related retinal thinning was also found on the edge of ILM peeling, where we started peeling with fine forceps. Conclusion: The ultrastructural studies showed that most of ILM peeling area was covered with glial cells during wound healing processes. Retinal changes were found comparable with dissociated optic nerve fiber layer appearance or dimple sign, which were clinically observed with optical coherence tomography.

Original languageEnglish
Pages (from-to)1523-1528
Number of pages6
JournalRetina
Volume37
Issue number8
DOIs
Publication statusPublished - Aug 1 2017

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Membranes
Nerve Fibers
Vitrectomy
Optic Nerve
Surgical Instruments
Retina
Scanning Transmission Electron Microscopy
Macaca fascicularis
Optical Coherence Tomography
Neuroglia
Wound Healing
Light

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

INCOMPLETE REPAIR of RETINAL STRUCTURE after VITRECTOMY with INTERNAL LIMITING MEMBRANE PEELING. / Hisatomi, Toshio; Tachibana, Takashi; Notomi, Shoji; Nakatake, Shunji; Fujiwara, Kohta; murakami, yusuke; Ikeda, Yasuhiro; Yoshida, Shigeo; Enaida, Hiroshi; Murata, Toshinori; Sakamoto, Taiji; Sonoda, Kohei; Ishibashi, Tatsuro.

In: Retina, Vol. 37, No. 8, 01.08.2017, p. 1523-1528.

Research output: Contribution to journalArticle

Hisatomi, T, Tachibana, T, Notomi, S, Nakatake, S, Fujiwara, K, murakami, Y, Ikeda, Y, Yoshida, S, Enaida, H, Murata, T, Sakamoto, T, Sonoda, K & Ishibashi, T 2017, 'INCOMPLETE REPAIR of RETINAL STRUCTURE after VITRECTOMY with INTERNAL LIMITING MEMBRANE PEELING', Retina, vol. 37, no. 8, pp. 1523-1528. https://doi.org/10.1097/IAE.0000000000001388
Hisatomi, Toshio ; Tachibana, Takashi ; Notomi, Shoji ; Nakatake, Shunji ; Fujiwara, Kohta ; murakami, yusuke ; Ikeda, Yasuhiro ; Yoshida, Shigeo ; Enaida, Hiroshi ; Murata, Toshinori ; Sakamoto, Taiji ; Sonoda, Kohei ; Ishibashi, Tatsuro. / INCOMPLETE REPAIR of RETINAL STRUCTURE after VITRECTOMY with INTERNAL LIMITING MEMBRANE PEELING. In: Retina. 2017 ; Vol. 37, No. 8. pp. 1523-1528.
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