Incomplete X-inactivation initiated by a hypomorphic Xist allele in the mouse

Yuko Hoki, Rieko Ikeda, Nathan Mise, Yuka Sakata, Tatsuya Ohhata, Hiroyuki Sasaki, Kuniya Abe, Takashi Sado

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    X chromosome inactivation (X-inactivation) in female mammals is triggered by differential upregulation of the Xist gene on one of the two X chromosomes and subsequent coating of the X in cis with its non-coding transcripts. Although targeted mutation has clearly shown that Xist is essential for X-inactivation in cis, the molecular mechanism by which Xist RNA induces chromosome silencing is largely unknown. Here, we demonstrate that an Xist mutant generated previously in mouse by gene targeting, XistIVS, is unique in that it partially retains the capacity to silence the X chromosome. Although XistIVS is differentially upregulated and its mutated transcript coats the X chromosome in cis in embryonic and extra-embryonic tissues, X-inactivation thus initiated does not seem to be fully established. The state of such incomplete inactivation is probably unstable and the mutated X is apparently reactivated in a subset of extra-embryonic tissues and, perhaps, early epiblastic cells. XistIVS, which can be referred to as a partial loss-of-function mutation, would provide an opportunity to dissect the molecular mechanism of Xist RNA-mediated chromosome silencing.

    Original languageEnglish
    Pages (from-to)2649-2659
    Number of pages11
    JournalDevelopment
    Volume138
    Issue number13
    DOIs
    Publication statusPublished - Jul 1 2011

    All Science Journal Classification (ASJC) codes

    • Molecular Biology
    • Developmental Biology

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