Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea

Kazunori Tanaka, Tasuku Shimizu, Muhammad Zakria, Joyce Njoloma, Yuichi Saeki, Masao Sakai, Takeo Yamakawa, Kiwamu Minamisawa, Shoichiro Akao

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Three endophytic bacterial strains (Burkholderia sp. No. 25, Enterobacter sp. No. 35 and Klebsiella sp. No. 38) isolated from sweet potato and sugarcane were examined for their ability to incorporate the gene encoding green fluorescent protein (GFP) via conjugation and electroporation. Enterobacter sp. No. 35 and Klebsiella sp. No. 38 were successfully tagged with the gfp gene by conjugation using pTn5kmgfpmutl. Fluorescence microscopic observation of Brassica oleracea inoculated with gfp-tagged endophytes revealed that Enterobacter sp. No. 35 and Klebsiella sp. No. 38 colonized the junctions between the lateral roots and the main roots.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalMicrobes and Environments
Volume21
Issue number2
DOIs
Publication statusPublished - Jan 1 2006

Fingerprint

colonizing ability
Enterobacter
Klebsiella
sweet potatoes
Brassica oleracea
green fluorescent protein
potato
sugarcane
nucleotide sequences
DNA
bacterium
protein
endophyte
gene
bacteria
Burkholderia
fluorescence
electroporation
endophytes
genes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

Cite this

Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea. / Tanaka, Kazunori; Shimizu, Tasuku; Zakria, Muhammad; Njoloma, Joyce; Saeki, Yuichi; Sakai, Masao; Yamakawa, Takeo; Minamisawa, Kiwamu; Akao, Shoichiro.

In: Microbes and Environments, Vol. 21, No. 2, 01.01.2006, p. 122-128.

Research output: Contribution to journalArticle

Tanaka, Kazunori ; Shimizu, Tasuku ; Zakria, Muhammad ; Njoloma, Joyce ; Saeki, Yuichi ; Sakai, Masao ; Yamakawa, Takeo ; Minamisawa, Kiwamu ; Akao, Shoichiro. / Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea. In: Microbes and Environments. 2006 ; Vol. 21, No. 2. pp. 122-128.
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