Incorporation of a DNA Sequence Encoding Green Fluorescent Protein (GFP) into Endophytic Diazotroph from Sugarcane and Sweet Potato and the Colonizing Ability of these Bacteria in Brassica oleracea

Kazunori Tanaka, Tasuku Shimizu, Muhammad Zakria, Joyce Njoloma, Yuichi Saeki, Masao Sakai, Takeo Yamakawa, Kiwamu Minamisawa, Shoichiro Akao

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Three endophytic bacterial strains (Burkholderia sp. No. 25, Enterobacter sp. No. 35 and Klebsiella sp. No. 38) isolated from sweet potato and sugarcane were examined for their ability to incorporate the gene encoding green fluorescent protein (GFP) via conjugation and electroporation. Enterobacter sp. No. 35 and Klebsiella sp. No. 38 were successfully tagged with the gfp gene by conjugation using pTn5kmgfpmutl. Fluorescence microscopic observation of Brassica oleracea inoculated with gfp-tagged endophytes revealed that Enterobacter sp. No. 35 and Klebsiella sp. No. 38 colonized the junctions between the lateral roots and the main roots.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalMicrobes and environments
Volume21
Issue number2
DOIs
Publication statusPublished - Jan 1 2006

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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