Incorporation of an inducible nucleotide analog into DNA by DNA polymerases

Md Monsur Ali, Shuhei Imoto, Yingfu Li, Shigeki Sasaki, Fumi Nagatsugi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5′-triphosphate of 2′-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis.

Original languageEnglish
Pages (from-to)2859-2863
Number of pages5
JournalBioorganic and Medicinal Chemistry
Volume17
Issue number7
DOIs
Publication statusPublished - Apr 1 2009

Fingerprint

DNA-Directed DNA Polymerase
Nucleotides
DNA
Oligonucleotides
Substrates
Research
2-amino-6-vinylpurine
purine
triphosphoric acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Incorporation of an inducible nucleotide analog into DNA by DNA polymerases. / Ali, Md Monsur; Imoto, Shuhei; Li, Yingfu; Sasaki, Shigeki; Nagatsugi, Fumi.

In: Bioorganic and Medicinal Chemistry, Vol. 17, No. 7, 01.04.2009, p. 2859-2863.

Research output: Contribution to journalArticle

Ali, Md Monsur ; Imoto, Shuhei ; Li, Yingfu ; Sasaki, Shigeki ; Nagatsugi, Fumi. / Incorporation of an inducible nucleotide analog into DNA by DNA polymerases. In: Bioorganic and Medicinal Chemistry. 2009 ; Vol. 17, No. 7. pp. 2859-2863.
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