Abstract
One of the major allenges in tissue engineering remains the construction of vascularized 3D transplants in vitro. We recently proposed novel technologies, termed "magnetic force-based tissue engineering" (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NEW sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NEW sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.
| Original language | English |
|---|---|
| Pages (from-to) | 51-58 |
| Number of pages | 8 |
| Journal | Journal of Chemical Engineering of Japan |
| Volume | 40 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 19 2007 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)
Cite this
Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering. / Ino, Kosuke; Ito, Akira; Kumazawa, Hirohito; Kagami, Hideaki; Ueda, Minoru; Honda, Hiroyuki.
In: Journal of Chemical Engineering of Japan, Vol. 40, No. 1, 19.01.2007, p. 51-58.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering
AU - Ino, Kosuke
AU - Ito, Akira
AU - Kumazawa, Hirohito
AU - Kagami, Hideaki
AU - Ueda, Minoru
AU - Honda, Hiroyuki
PY - 2007/1/19
Y1 - 2007/1/19
N2 - One of the major allenges in tissue engineering remains the construction of vascularized 3D transplants in vitro. We recently proposed novel technologies, termed "magnetic force-based tissue engineering" (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NEW sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NEW sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.
AB - One of the major allenges in tissue engineering remains the construction of vascularized 3D transplants in vitro. We recently proposed novel technologies, termed "magnetic force-based tissue engineering" (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NEW sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NEW sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.
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U2 - 10.1252/jcej.40.51
DO - 10.1252/jcej.40.51
M3 - Article
VL - 40
SP - 51
EP - 58
JO - Journal of Chemical Engineering of Japan
JF - Journal of Chemical Engineering of Japan
SN - 0021-9592
IS - 1
ER -