Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle

Akihito Harada, Kazumitsu Maehara, Yuko Sato, Daijiro Konno, Taro Tachibana, Hiroshi Kimura, Yasuyuki Ohkawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Lineage potential is triggered by lineage-specific transcription factors in association with changes in the chromatin structure. Histone H3.3 variant is thought to play an important role in the regulation of lineage-specific genes. To elucidate the function of H3.3 in myogenic differentiation, we forced the expression of GFP-H3.1 to alter the balance between H3.1 and H3.3 in mouse C2C12 cells that could be differentiated into myotubes. GFP-H3.1 replaced H3.3 in the regulatory regions of skeletal muscle (SKM) genes and induced a decrease of H3K4 trimethylation (H3K4me3) and increase of H3K27 trimethylation (H3K27me3). Similar results were obtained by H3.3 knockdown. In contrast, MyoD-dependent H3.3 incorporation into SKM genes in fibroblasts induced an increase of H3K4me3 and H3K27me3. In mouse embryos, a bivalent modification of H3K4me3 and H3K27me3 was formed on H3.3-incorporated SKM genes before embryonic skeletal muscle differentiation. These results suggest that lineage potential is established through a selective incorporation of specific H3 variants that governs the balance of histone modifications.

Original languageEnglish
Pages (from-to)775-786
Number of pages12
JournalNucleic acids research
Volume43
Issue number2
DOIs
Publication statusPublished - Jan 30 2015

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Histones
Skeletal Muscle
Genes
Histone Code
Nucleic Acid Regulatory Sequences
Skeletal Muscle Fibers
Chromatin
Transcription Factors
Embryonic Structures
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle. / Harada, Akihito; Maehara, Kazumitsu; Sato, Yuko; Konno, Daijiro; Tachibana, Taro; Kimura, Hiroshi; Ohkawa, Yasuyuki.

In: Nucleic acids research, Vol. 43, No. 2, 30.01.2015, p. 775-786.

Research output: Contribution to journalArticle

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