Increase in calcium in smooth muscle cells of the rabbit bladder induced by acetylcholine and ATP

Masahiro Oike, Kate E. Creed, Hitoshi Onoue, Hiroyuki Tanaka, Yushi Ito

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cultured smooth muscle cells from rabbit urinary bladder were loaded with fura-2. Changes in intracellular Ca concentration [Ca 2+ ](i) produced by acetylcholine (ACh) or adenosine triphosphate (ATP) were estimated by measuring the fluorescence ratio F340/F380. Western blot analysis and immunohistochemical techniques showed that the cultured cells retained α- smooth muscle actin. ATP produced a rapid but transient increase in [Ca 2+ ](i) and ACh produced a delayed, prolonged increase. Application of ACh after ATP in Ca-free solution failed to elevate [Ca 2+ ](i) suggesting that both ACh and ATP release Ca 2+ from the same intracellular stores. Following application of ACh but not ATP in Ca-free Krebs solution, reintroduction of Ca 2+ produced elevation of [Ca 2+ ](i), indicating that ACh causes prolonged opening of channels in the membrane. The sustained increase induced by ACh was abolished by nicardipine (blocker of Ca 2+ voltage dependent channel I(Ca(V))) or quinine (blocker of non-selective cation channels). Although the elevations to ACh or ATP were abolished by neomycin (an inbibitor of phospholipase C) the different time courses suggest that the mechanisms of release of Ca 2+ from intracellular stores or the pathway for refilling the stores is different.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalJournal of the Autonomic Nervous System
Volume69
Issue number2-3
DOIs
Publication statusPublished - Apr 30 1998

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Acetylcholine
Smooth Muscle Myocytes
Urinary Bladder
Adenosine Triphosphate
Rabbits
Calcium
Nicardipine
Neomycin
Quinine
Fura-2
Type C Phospholipases
Ion Channels
Smooth Muscle
Cations
Actins
Cultured Cells
Fluorescence
Western Blotting

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology
  • Clinical Neurology

Cite this

Increase in calcium in smooth muscle cells of the rabbit bladder induced by acetylcholine and ATP. / Oike, Masahiro; Creed, Kate E.; Onoue, Hitoshi; Tanaka, Hiroyuki; Ito, Yushi.

In: Journal of the Autonomic Nervous System, Vol. 69, No. 2-3, 30.04.1998, p. 141-147.

Research output: Contribution to journalArticle

Oike, Masahiro ; Creed, Kate E. ; Onoue, Hitoshi ; Tanaka, Hiroyuki ; Ito, Yushi. / Increase in calcium in smooth muscle cells of the rabbit bladder induced by acetylcholine and ATP. In: Journal of the Autonomic Nervous System. 1998 ; Vol. 69, No. 2-3. pp. 141-147.
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