Abstract
Hydrocortisone was investigated for its ability to differentiate human leukemia KU812 cells into mature hematopoietic cells including basophils. Hydrocortisone treatment increased the amount of intracellular histamine by up-regulation of L-histidine decarboxylase (HDC) mRNA and enhanced cell surface expression of the high affinity IgE receptor FcεRI. Histamine is catalyzed from L-histidine by HDC, which in blood cell types is only expressed in basophils and mast cells. Cells, on which the FcεRI expression was enhanced by hydrocortisone, were shown to release histamine when stimulated with anti-IgE antibody after sensitization with myeloma IgE, implying that the induced FcεRI molecules were able to transduce a signal for degranulation. These results suggest that hydrocortisone promotes differentiation of KU812 cells into functionally mature basophilic cells.
| Original language | English |
|---|---|
| Pages (from-to) | 213-223 |
| Number of pages | 11 |
| Journal | Cytotechnology |
| Volume | 34 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 2000 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Clinical Biochemistry
- Cell Biology
Cite this
Increase in histamine content and enhancement of high affinity IgE receptor FcεRI expression in the human leukemia KU812 cells upon treatment with hydrocortisone. / Hara, Takashi; Tachibana, Hirofumi; Yamada, Koji.
In: Cytotechnology, Vol. 34, No. 3, 01.01.2000, p. 213-223.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increase in histamine content and enhancement of high affinity IgE receptor FcεRI expression in the human leukemia KU812 cells upon treatment with hydrocortisone
AU - Hara, Takashi
AU - Tachibana, Hirofumi
AU - Yamada, Koji
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Hydrocortisone was investigated for its ability to differentiate human leukemia KU812 cells into mature hematopoietic cells including basophils. Hydrocortisone treatment increased the amount of intracellular histamine by up-regulation of L-histidine decarboxylase (HDC) mRNA and enhanced cell surface expression of the high affinity IgE receptor FcεRI. Histamine is catalyzed from L-histidine by HDC, which in blood cell types is only expressed in basophils and mast cells. Cells, on which the FcεRI expression was enhanced by hydrocortisone, were shown to release histamine when stimulated with anti-IgE antibody after sensitization with myeloma IgE, implying that the induced FcεRI molecules were able to transduce a signal for degranulation. These results suggest that hydrocortisone promotes differentiation of KU812 cells into functionally mature basophilic cells.
AB - Hydrocortisone was investigated for its ability to differentiate human leukemia KU812 cells into mature hematopoietic cells including basophils. Hydrocortisone treatment increased the amount of intracellular histamine by up-regulation of L-histidine decarboxylase (HDC) mRNA and enhanced cell surface expression of the high affinity IgE receptor FcεRI. Histamine is catalyzed from L-histidine by HDC, which in blood cell types is only expressed in basophils and mast cells. Cells, on which the FcεRI expression was enhanced by hydrocortisone, were shown to release histamine when stimulated with anti-IgE antibody after sensitization with myeloma IgE, implying that the induced FcεRI molecules were able to transduce a signal for degranulation. These results suggest that hydrocortisone promotes differentiation of KU812 cells into functionally mature basophilic cells.
UR - http://www.scopus.com/inward/record.url?scp=0034462166&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034462166&partnerID=8YFLogxK
U2 - 10.1023/A:1008183400709
DO - 10.1023/A:1008183400709
M3 - Article
C2 - 19003397
AN - SCOPUS:0034462166
VL - 34
SP - 213
EP - 223
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 3
ER -