Increase in histamine content and enhancement of high affinity IgE receptor FcεRI expression in the human leukemia KU812 cells upon treatment with hydrocortisone

Takashi Hara, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hydrocortisone was investigated for its ability to differentiate human leukemia KU812 cells into mature hematopoietic cells including basophils. Hydrocortisone treatment increased the amount of intracellular histamine by up-regulation of L-histidine decarboxylase (HDC) mRNA and enhanced cell surface expression of the high affinity IgE receptor FcεRI. Histamine is catalyzed from L-histidine by HDC, which in blood cell types is only expressed in basophils and mast cells. Cells, on which the FcεRI expression was enhanced by hydrocortisone, were shown to release histamine when stimulated with anti-IgE antibody after sensitization with myeloma IgE, implying that the induced FcεRI molecules were able to transduce a signal for degranulation. These results suggest that hydrocortisone promotes differentiation of KU812 cells into functionally mature basophilic cells.

Original languageEnglish
Pages (from-to)213-223
Number of pages11
JournalCytotechnology
Volume34
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

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Cortisol
IgE Receptors
Histamine
Hydrocortisone
Leukemia
Histidine
Histidine Decarboxylase
Basophils
Antibodies
Immunoglobulin E
Histamine Release
Blood
Mast Cells
Cells
Cell Differentiation
Blood Cells
Up-Regulation
Messenger RNA
Molecules

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

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