Increase in resistance to extended-spectrum cephalosporins in Salmonella isolated from retail chicken products in Japan

Tamie Noda, Koichi Murakami, Yoshiki Etoh, Fuyuki Okamoto, Jun Yatsuyanagi, Nobuyuki Sera, Munenori Furuta, Daisuke Onozuka, Takahiro Oda, Tetsuo Asai, Shuji Fujimoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Extended-spectrum β-lactamase (ESBL)-producing Salmonella are one of the most important public health problems in developed countries. ESBL-producing Salmonella strains have been isolated from humans in Asian countries neighboring Japan, along with strains harboring the plasmid-mediated extended-spectrum cephalosporin (ESC)-resistance gene, ampC (pAmpC). However, only a few studies have investigated the prevalence of ESC-resistant Salmonella in chicken products in Japan, which are the main vehicle of Salmonella transmission. The aim of this study was to investigate the prevalence of ESBL-producing, pAmpC-harboring, or carbapenem-resistant Salmonella in chicken products in Japan. In total, 355 out of 779 (45.6%) chicken product samples collected from 1996-2010 contained Salmonella, resulting in 378 distinct isolates. Of these isolates, 373 were tested for resistance to ESCs, cephamycins, or carbapenems. Isolates that showed resistance to one or more of these antimicrobials were then examined by PCR and DNA sequence analysis for the presence of the blaCMY, blaCTX-M, blaTEM, and blaSHV resistance genes. Thirty-five resistant isolates were detected, including 26 isolates that contained pAmpC (blaCMY-2), and nine ESBL-producing isolates harboring blaCTX-M (n = 4, consisting of two blaCTX-M-2 and two blaCTX-M-15 genes), blaTEM (n = 4, consisting of one blaTEM-20 and three blaTEM-52 genes), and blaSHV(n = 1, blaSHV-12). All pAmpC-harboring and ESBL-producing Salmonella isolates were obtained from samples collected after 2005, and the percentage of resistant isolates increased significantly from 0% in 2004 to 27.9% in 2010 (P for trend = 0.006). This increase was caused in part by an increase in the number of Salmonella enterica subsp. enterica serovar Infantis strains harboring an approximately 280-kb plasmid containing blaCMY-2 in proximity to ISEcp1. The dissemination of ESC-resistant Salmonella containing plasmid-mediated blaCMY-2 in chicken products indicates the need for the development of continuous monitoring strategies in the interests of public health.

Original languageEnglish
Article numbere0116927
JournalPloS one
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 2 2015

Fingerprint

cephalosporins
Salmonella
Cephalosporins
Chickens
Japan
chickens
carbapenems
Genes
plasmids
Carbapenems
Plasmids
Public health
cephamycins
public health
genes
Cephalosporin Resistance
Public Health
Cephamycins
Salmonella Infantis
Salmonella enterica

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Noda, T., Murakami, K., Etoh, Y., Okamoto, F., Yatsuyanagi, J., Sera, N., ... Fujimoto, S. (2015). Increase in resistance to extended-spectrum cephalosporins in Salmonella isolated from retail chicken products in Japan. PloS one, 10(2), [e0116927]. https://doi.org/10.1371/journal.pone.0116927

Increase in resistance to extended-spectrum cephalosporins in Salmonella isolated from retail chicken products in Japan. / Noda, Tamie; Murakami, Koichi; Etoh, Yoshiki; Okamoto, Fuyuki; Yatsuyanagi, Jun; Sera, Nobuyuki; Furuta, Munenori; Onozuka, Daisuke; Oda, Takahiro; Asai, Tetsuo; Fujimoto, Shuji.

In: PloS one, Vol. 10, No. 2, e0116927, 02.02.2015.

Research output: Contribution to journalArticle

Noda, T, Murakami, K, Etoh, Y, Okamoto, F, Yatsuyanagi, J, Sera, N, Furuta, M, Onozuka, D, Oda, T, Asai, T & Fujimoto, S 2015, 'Increase in resistance to extended-spectrum cephalosporins in Salmonella isolated from retail chicken products in Japan', PloS one, vol. 10, no. 2, e0116927. https://doi.org/10.1371/journal.pone.0116927
Noda, Tamie ; Murakami, Koichi ; Etoh, Yoshiki ; Okamoto, Fuyuki ; Yatsuyanagi, Jun ; Sera, Nobuyuki ; Furuta, Munenori ; Onozuka, Daisuke ; Oda, Takahiro ; Asai, Tetsuo ; Fujimoto, Shuji. / Increase in resistance to extended-spectrum cephalosporins in Salmonella isolated from retail chicken products in Japan. In: PloS one. 2015 ; Vol. 10, No. 2.
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