Increase of immunoglobulin productivity of human-human hybridoma HB4C5 cells by histone

Takeaki Okamoto, Takuya Sugahara, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A histone mixture (H1, H2A, H2B, H3, and H4) derived from calf thymus stimulated IgM production by human-human hybridoma HB4C5 cells. On the contrary, the histone mixture did not increase IgM production by the human Burkitt's lymphoma cell line NAT-30, IgG production by the human B lymphoblastoid cell line HMy-2, and IgE production by the human myeloma cell line U266. The immunoglobulin production-stimulating activity of the histone mixture was inactivated by trypsin or chymotrypsin digestion. In addition, confocal laser microscopic analysis had shown that HB4C5 cells incorporated a lot of histone but other cell lines did not incorporate it as much. These facts strongly suggest that histone acts as an immunoglobulin production-stimulating factor (IPSF) after internalization into the human B cell lines and the native structure of histone is required for the IPSF activity.

Original languageEnglish
Pages (from-to)1241-1245
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number6
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Hybridomas
Histones
Immunoglobulins
Productivity
Cell Line
Cells
Immunoglobulin M
Burkitt Lymphoma
Thymus
Chymotrypsin
Thymus Gland
Immunoglobulin E
Digestion
Lasers
Trypsin
B-Lymphocytes
Immunoglobulin G

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Increase of immunoglobulin productivity of human-human hybridoma HB4C5 cells by histone. / Okamoto, Takeaki; Sugahara, Takuya; Tachibana, Hirofumi; Yamada, Koji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 66, No. 6, 01.01.2002, p. 1241-1245.

Research output: Contribution to journalArticle

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