TY - JOUR
T1 - Increase of peripheral B lymphocytes committed to the production of monoreactive and high affinity antibodies to HTLV-1 in patients with HAM/TSP
AU - Nakamura, Minoru
AU - Itoyama, Yasuto
AU - Kuroki, Mika
AU - Nakano, Shuji
AU - Kondoh, Seiji
AU - Nagafuchi, Seiho
AU - Kira, Jun ichi
AU - Ichinose, Ichiro
AU - Mitsugi, Kenji
AU - Anzai, Keizo
AU - Mori, Hideharu
AU - Fukui, Masanori
AU - Okamura, Seiichi
AU - Niho, Yoshiyuki
N1 - Funding Information:
We thank Dr. S. Kashiwagi and Dr. J. Hayashi (The Department of General Medicine, Kyushu University) for providing blood samples and Dr. H. Shiraki (Fukuoka Red Cross Blood Center, Fukuoka 818, Japan) for providing synthetic peptides of HTLV-1 envelope glycoproteins. This work was supported in part by a grand-in-aid for the encouragement of young scientists, from the Ministry of Education, Science, and Culture of Japan, and grants from Fukuoka Cancer Society, Rinsho Igaku Shinko Zaidan and Uehara Zaidan.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/3
Y1 - 1992/3
N2 - We quantitated the frequency of B lymphocytes capable of producing antibodies to HTLV-1 in the peripheral blood from patients with HAM/TSP, non-HAM/TSP HTLV-1 carriers and seronegative healthy subjects. Epstein-Barr virus (EBV) was used as a polyclonal activator of B lymphocytes in a limiting dilution condition. We found that B lymphocytes committed to the production of monoreactive-IgG and -IgA antibodies to recombinant HTLV-1 (gag + env) hybrid protein were significantly increased in a number in patients with HAM/TSP as compared to non-HAM/TSP HTLV-1 carriers and seronegative healthy subjects. By transforming these B lymphocytes with EBV and fusing them with human-mouse heteromyeloma (F3B6), a stable hybridoma producing IgG monoclonal antibody (mAb) to HTLV-1 (gag + env) protein was generated from a patient with HAM/TSP. This mAb (IgG1, κ), designated F31.1, specifically bound to the amino acid residues from 235 to 254 of HTLV-1 envelope glycoproteins (gp46) with high affinity (Kd = 4.0 × 10-9 mol/1). These data indicate that the antigen-driven process of B lymphocytes maturation by HTLV-1 antigens is markedly increased in patients with HAM/TSP.
AB - We quantitated the frequency of B lymphocytes capable of producing antibodies to HTLV-1 in the peripheral blood from patients with HAM/TSP, non-HAM/TSP HTLV-1 carriers and seronegative healthy subjects. Epstein-Barr virus (EBV) was used as a polyclonal activator of B lymphocytes in a limiting dilution condition. We found that B lymphocytes committed to the production of monoreactive-IgG and -IgA antibodies to recombinant HTLV-1 (gag + env) hybrid protein were significantly increased in a number in patients with HAM/TSP as compared to non-HAM/TSP HTLV-1 carriers and seronegative healthy subjects. By transforming these B lymphocytes with EBV and fusing them with human-mouse heteromyeloma (F3B6), a stable hybridoma producing IgG monoclonal antibody (mAb) to HTLV-1 (gag + env) protein was generated from a patient with HAM/TSP. This mAb (IgG1, κ), designated F31.1, specifically bound to the amino acid residues from 235 to 254 of HTLV-1 envelope glycoproteins (gp46) with high affinity (Kd = 4.0 × 10-9 mol/1). These data indicate that the antigen-driven process of B lymphocytes maturation by HTLV-1 antigens is markedly increased in patients with HAM/TSP.
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U2 - 10.1016/0165-5728(92)90153-C
DO - 10.1016/0165-5728(92)90153-C
M3 - Article
C2 - 1372329
AN - SCOPUS:0026584408
VL - 37
SP - 35
EP - 45
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -
