Increase of RP105-1acking activated B cells in the peripheral blood and salivary glands in patients with Sjögren's syndrome

Yuji Kikuchi, Syuichi Koarada, Seiji Nakamura, Nobuhisa Yonemitsu, Yoshifumi Tada, Yoshio Haruta, Fumitaka Morito, Akihide Ohta, Kensuke Miyake, Takahiko Horiuchi, Kohei Nagasawa

Research output: Contribution to journalArticle

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Abstract

Objective: To quantify the activated B cells in the peripheral blood and salivary glands of patients with Sjögren's syndrome (SS) by analyzing the expression of RP105 molecule on the B cells. Methods: The expression of RP105 on the peripheral blood B cells of patients with SS (19 cases) was analyzed by flow cytometry. RP105-positive and negative B cells were sorted and cultured in vitro and the amount of immunoglobulins (IgG and IgM) produced in the supernatant was measured by enzyme-linked immunosorbent assay (ELISA). Salivary gland biopsy samples from 9 SS patients were histologically evaluated and the sequential frozen sections were separately immunostained by anti-RP105 and anti-CD20 monoclonal antibodies. Results: A significantly higher proportion of peripheral blood RP105-negative B cells was found in SS patients than in healthy individuals. RP105-negative, but not positive, B cells from SS patients were capable of producing IgG and IgM spontaneously in vitro, which was enhanced by the addition of Staphylococcus aureus Cowan I strain (SAC) or IL-6. Salivary glands from 2 of 9 SS patients were found to have lymphoid follicles whose germinal centers consisted of RP105-negative B cells. Moreover, a larger proportion of B cells extensively infiltrating the area other than lymphoid follicles was also RP105-negative. Conclusion: RP105-negative B cells, a subset of highly activated and well differentiated B cells, which are increased in number in the peripheral blood and extensively infiltrate salivary glands, may be responsible for the production of class-switched immunoglobulin in SS. In addition, those cells might be associated with the inflammation and tissue damage of the salivary glands.

Original languageEnglish
Pages (from-to)5-12
Number of pages8
JournalClinical and experimental rheumatology
Volume26
Issue number1
Publication statusPublished - Jan 1 2008

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Salivary Glands
B-Lymphocytes
Immunoglobulin M
Immunoglobulin G
B-Lymphocyte Subsets
Germinal Center
Immunoglobulin Isotypes
Frozen Sections
Staphylococcus aureus
Immunoglobulins
Interleukin-6
Blood Cells
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Inflammation
Biopsy

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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Increase of RP105-1acking activated B cells in the peripheral blood and salivary glands in patients with Sjögren's syndrome. / Kikuchi, Yuji; Koarada, Syuichi; Nakamura, Seiji; Yonemitsu, Nobuhisa; Tada, Yoshifumi; Haruta, Yoshio; Morito, Fumitaka; Ohta, Akihide; Miyake, Kensuke; Horiuchi, Takahiko; Nagasawa, Kohei.

In: Clinical and experimental rheumatology, Vol. 26, No. 1, 01.01.2008, p. 5-12.

Research output: Contribution to journalArticle

Kikuchi, Y, Koarada, S, Nakamura, S, Yonemitsu, N, Tada, Y, Haruta, Y, Morito, F, Ohta, A, Miyake, K, Horiuchi, T & Nagasawa, K 2008, 'Increase of RP105-1acking activated B cells in the peripheral blood and salivary glands in patients with Sjögren's syndrome', Clinical and experimental rheumatology, vol. 26, no. 1, pp. 5-12.
Kikuchi, Yuji ; Koarada, Syuichi ; Nakamura, Seiji ; Yonemitsu, Nobuhisa ; Tada, Yoshifumi ; Haruta, Yoshio ; Morito, Fumitaka ; Ohta, Akihide ; Miyake, Kensuke ; Horiuchi, Takahiko ; Nagasawa, Kohei. / Increase of RP105-1acking activated B cells in the peripheral blood and salivary glands in patients with Sjögren's syndrome. In: Clinical and experimental rheumatology. 2008 ; Vol. 26, No. 1. pp. 5-12.
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AU - Morito, Fumitaka

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