Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death

Takashi Kadono, Daniel Tran, Rafik Errakhi, Takuya Hiramatsu, Patrice Meimoun, Joël Briand, Mari Iwaya-Inoue, Tomonori Kawano, François Bouteau

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background: Ozone is a major secondary air pollutant often reaching high concentrations in urban areas under strong daylight, high temperature and stagnant high-pressure systems. Ozone in the troposphere is a pollutant that is harmful to the plant. Principal Findings: By exposing cells to a strong pulse of ozonized air, an acute cell death was observed in suspension cells of Arabidopsis thaliana used as a model. We demonstrated that O3 treatment induced the activation of a plasma membrane anion channel that is an early prerequisite of O3-induced cell death in A. thaliana. Our data further suggest interplay of anion channel activation with well known plant responses to O3, Ca2+ influx and NADPH-oxidase generated reactive oxygen species (ROS) in mediating the oxidative cell death. This interplay might be fuelled by several mechanisms in addition to the direct ROS generation by O3; namely, H2O2 generation by salicylic and abscisic acids. Anion channel activation was also shown to promote the accumulation of transcripts encoding vacuolar processing enzymes, a family of proteases previously reported to contribute to the disruption of vacuole integrity observed during programmed cell death. Significance: Collectively, our data indicate that anion efflux is an early key component of morphological and biochemical events leading to O3-induced programmed cell death. Because ion channels and more specifically anion channels assume a crucial position in cells, an understanding about the underlying role(s) for ion channels in the signalling pathway leading to programmed cell death is a subject that warrants future investigation.

Original languageEnglish
Article numbere13373
JournalPLoS One
Volume5
Issue number10
DOIs
Publication statusPublished - Oct 13 2010

Fingerprint

Ozone
Cell death
ozone
anions
Anions
Cell Death
apoptosis
cell death
Ion Channels
ion channels
Chemical activation
reactive oxygen species
Arabidopsis
Arabidopsis thaliana
pollutants
Reactive Oxygen Species
air
Air Pollutants
Abscisic Acid
Troposphere

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kadono, T., Tran, D., Errakhi, R., Hiramatsu, T., Meimoun, P., Briand, J., ... Bouteau, F. (2010). Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death. PLoS One, 5(10), [e13373]. https://doi.org/10.1371/journal.pone.0013373

Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death. / Kadono, Takashi; Tran, Daniel; Errakhi, Rafik; Hiramatsu, Takuya; Meimoun, Patrice; Briand, Joël; Iwaya-Inoue, Mari; Kawano, Tomonori; Bouteau, François.

In: PLoS One, Vol. 5, No. 10, e13373, 13.10.2010.

Research output: Contribution to journalArticle

Kadono, T, Tran, D, Errakhi, R, Hiramatsu, T, Meimoun, P, Briand, J, Iwaya-Inoue, M, Kawano, T & Bouteau, F 2010, 'Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death', PLoS One, vol. 5, no. 10, e13373. https://doi.org/10.1371/journal.pone.0013373
Kadono T, Tran D, Errakhi R, Hiramatsu T, Meimoun P, Briand J et al. Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death. PLoS One. 2010 Oct 13;5(10). e13373. https://doi.org/10.1371/journal.pone.0013373
Kadono, Takashi ; Tran, Daniel ; Errakhi, Rafik ; Hiramatsu, Takuya ; Meimoun, Patrice ; Briand, Joël ; Iwaya-Inoue, Mari ; Kawano, Tomonori ; Bouteau, François. / Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death. In: PLoS One. 2010 ; Vol. 5, No. 10.
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