Increased biofilm formation ability and accelerated transport of Staphylococcus aureus along a catheter during reciprocal movements

Isao Haraga, Shintaro Abe, Shiro Jimi, Fumiaki Kiyomi, Ken Yamaura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Staphylococcus spp. is a major cause of device-related infections. However, the mechanisms of deep-tissue infection by staphylococci from the skin surface remain unclear. We performed in vitro experiments to determine how staphylococci are transferred from the surface to the deeper layers of agar along the catheter for different strains of Staphylococcus aureus with respect to bacterial concentrations, catheter movements, and biofilm formation. We found that when 5-mm reciprocal movements of the catheter were repeated every 8 h, all catheter samples of S. aureus penetrated the typical distance of 50 mm from the skin to the epidural space. The number of reciprocal catheter movements and the depth of bacterial growth were correlated. A greater regression coefficient for different strains implied faster bacterial growth. Enhanced biofilm formation by different strains implied larger regression coefficients. Increased biofilm formation ability may accelerate S. aureus transport along a catheter due to physical movements by patients.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalJournal of Microbiological Methods
Volume132
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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