Increased expression of ADAM 9 and ADAM 15 mRNA in pancreatic cancer

Daisuke Yamada, Kenoki Ohuchida, Kazuhiro Mizumoto, Seiji Ohhashi, Jun Yu, Takuya Egami, Hayato Fujita, Eishi Nagai, Masao Tanaka

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Background: A disintegrin and metalloproteases (ADAMs) comprise a multifunctional family of membrane-anchored proteins. ADAM 9 and ADAM 15 are involved in cell migration and invasion. Expression of ADAM 9 and ADAM 15 was reported to be altered in several types of cancer. Materials and Methods: Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure the expression of ADAM 9 mRNA in bulk pancreatic tissues. Results showed no significant difference in the expression of ADAM 9 mRNA between pancreatic cancer and non-neoplastic pancreas. Primary cultured pancreatic fibroblasts also expressed ADAM 9 mRNA. Therefore, a laser microdissection and pressure catapulting technique was employed to isolate cancer cells from tumor tissues. The expression of ADAM 9 and ADAM 15 mRNA was measured in microdissected samples (cancer cells, n=11; normal epithelial cells, n=13 for ADAM 9; cancer cells, n=9; normal epithelial cells, n=9 for ADAM 15). Results: Pancreatic cancer cells expressed significantly higher levels of ADAM 9 and ADAM 15 mRNA than did normal pancreatic epithelial cells (p=0.016 for ADAM 9; p=0.004 for ADAM 15). Conclusion: ADAM 9 and ADAM 15 are involved in pancreatic cancer. Microdissection-based analysis appears to be indispensable for the accurate analysis of the expression of certain ADAM family members in pancreatic cancer.

Original languageEnglish
Pages (from-to)793-799
Number of pages7
JournalAnticancer research
Volume27
Issue number2
Publication statusPublished - Mar 2007

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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