Increased reactive oxygen species in rostral ventrolateral medulla contribute to neural mechanisms of hypertension in stroke-prone spontaneously hypertensive rats

Takuya Kishi, Yoshitaka Hirooka, Yoshikuni Kimura, Koji Ito, Hiroaki Shimokawa, Akira Takeshita

Research output: Contribution to journalArticle

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Abstract

Background - Oxidative stress increases in hypertension. The aim of this study was to determine whether reactive oxygen species (ROS) are increased in the rostral ventrolateral medulla (RVLM) in the brainstem, where the vasomotor center is located, in stroke-prone spontaneously hypertensive rats (SHRSP), and, if so, to determine whether the increased ROS contribute to neural mechanisms of hypertension in SHRSP. Methods and Results - We measured ROS levels in the RVLM of SHRSP and compared them with those in Wistar-Kyoto rats (WKY). Thiobarbituric acid-reactive substances were increased in SHRSP compared with WKY. ROS were measured by electron spin resonance (ESR) spectroscopy. The ESR signal decay rate in the RVLM of SHRSP was significantly increased compared with that in WKY, and this increase was abolished by dimethylthiourea (a hydroxyl radical scavenger). The increased ESR signal decay rate was reduced to the same extent in the presence of desferrioxamine, catalase, and Tiron, indicating that hydroxyl radicals are derived from superoxide anions and hydrogen peroxide. In addition, total superoxide dismutase (SOD) activity in the RVLM was decreased in SHRSP compared with WKY. Furthermore, bilateral microinjection of tempol into the RVLM decreased blood pressure in SHRSP but not in WKY, and MnSOD overexpression in the RVLM of SHRSP decreased blood pressure and inhibited sympathetic nerve activity. Conclusions - These results suggest that superoxide anions in the RVLM, which generate hydroxyl radicals, are increased in SHRSP and contribute to the neural mechanisms of hypertension in SHRSP.

Original languageEnglish
Pages (from-to)2357-2362
Number of pages6
JournalCirculation
Volume109
Issue number19
DOIs
Publication statusPublished - May 18 2004

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Inbred WKY Rats
Inbred SHR Rats
Reactive Oxygen Species
Stroke
Hypertension
Electron Spin Resonance Spectroscopy
Hydroxyl Radical
Superoxides
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
Blood Pressure
Deferoxamine
Thiobarbituric Acid Reactive Substances
Microinjections
Catalase
Hydrogen Peroxide
Brain Stem
Superoxide Dismutase
Oxidative Stress

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Increased reactive oxygen species in rostral ventrolateral medulla contribute to neural mechanisms of hypertension in stroke-prone spontaneously hypertensive rats. / Kishi, Takuya; Hirooka, Yoshitaka; Kimura, Yoshikuni; Ito, Koji; Shimokawa, Hiroaki; Takeshita, Akira.

In: Circulation, Vol. 109, No. 19, 18.05.2004, p. 2357-2362.

Research output: Contribution to journalArticle

Kishi, Takuya ; Hirooka, Yoshitaka ; Kimura, Yoshikuni ; Ito, Koji ; Shimokawa, Hiroaki ; Takeshita, Akira. / Increased reactive oxygen species in rostral ventrolateral medulla contribute to neural mechanisms of hypertension in stroke-prone spontaneously hypertensive rats. In: Circulation. 2004 ; Vol. 109, No. 19. pp. 2357-2362.
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