Increased serum oxysterol concentrations in patients with chronic hepatitis C virus infection

Tadashi Ikegami, Akira Honda, Teruo Miyazaki, Motoyuki Kohjima, Makoto Nakamuta, Yasushi Matsuzaki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Oxidative stress and dysregulated cholesterol metabolism are characteristic features of chronic hepatitis C virus infection (CHC). Therefore, we analyzed serum oxysterol profiles in CHC patients and examined the significance of oxysterols in CHC. The concentrations of 7α-hydroxycholesterol, 4β-hydroxycholesterol and 25-hydroxycholesterol as determined by LC-ESI-MS/MS were significantly elevated by +236%, +29% and +44%, respectively, in CHC patients compared with controls. Moreover, the elevated levels were significantly decreased by anti-viral therapy using PEGylated-interferon and ribavirin for 3 months. In contrast, 24S-hydroxycholesterol, 27-hydroxycholesterol and 7α-hydroxy-4-cholesten-3-one concentrations were not affected by CHC or anti-viral treatment. These results suggest that some oxysterols that are elevated in CHC are produced by cholesterol autoxidation due to oxidative stress or inflammation in the liver. Oxysterols may represent novel targets for the inhibition of disease progression and the prevention of hepatocarcinogenesis in CHC patients.

Original languageEnglish
Pages (from-to)736-740
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume446
Issue number3
DOIs
Publication statusPublished - Apr 11 2014
Externally publishedYes

Fingerprint

Oxidative stress
Chronic Hepatitis C
Virus Diseases
Viruses
Hepacivirus
Cholesterol
Ribavirin
Serum
Metabolism
Liver
Interferons
Oxidative Stress
Hydroxycholesterols
Disease Progression
Inflammation
Oxysterols
Therapeutics
7 alpha-hydroxy-4-cholesten-3-one
27-hydroxycholesterol
cholest-5-ene-3,4-diol

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Increased serum oxysterol concentrations in patients with chronic hepatitis C virus infection. / Ikegami, Tadashi; Honda, Akira; Miyazaki, Teruo; Kohjima, Motoyuki; Nakamuta, Makoto; Matsuzaki, Yasushi.

In: Biochemical and Biophysical Research Communications, Vol. 446, No. 3, 11.04.2014, p. 736-740.

Research output: Contribution to journalArticle

Ikegami, Tadashi ; Honda, Akira ; Miyazaki, Teruo ; Kohjima, Motoyuki ; Nakamuta, Makoto ; Matsuzaki, Yasushi. / Increased serum oxysterol concentrations in patients with chronic hepatitis C virus infection. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 446, No. 3. pp. 736-740.
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