Increased Susceptibility to Escherichia coli Infection in Mice Pretreated with Corynebacterium parvum

Yasunobu Yoshikai, Shunji Miake, Masatoshi Sano, Kikuo Nomoto

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The contribution of activated macrophages to protection against Escherichia coli was studied in mice treated intravenously with Corynebacterium parvum 7 days before infection. C. parvum‐treated mice showed increased phagocytic activity and enhanced resistance to Listeria infection. In contrast, these mice showed increased susceptibility to a subsequent challenge with E. coli that correlated closely with a reduction in the LD50 of lipopolysaccharide (LPS) in these mice. The peritoneal macrophages obtained from C. parvum‐treated mice had a strong ability to phagocytize and kill E. coli in in vitro experiments. A rapid decline in the number of bacteria in the liver of C. parvum‐treated mice was observed in the early period of infection. However, the number of bacteria in liver and spleen increased progressively to a lethal dose from 6 hr after infection. At this time, a significant increase in β‐glucuronidase, a lysosomal acid hydrolase, was found in the serum of these mice. In vitro experiments revealed that the peritoneal macrophages from C. parvum‐treated mice were highly susceptible to the cytotoxic effect of LPS after 6 hr of incubation with LPS. It is suggested that the hypersensitivity of activated macrophages to the cytotoxic effect of endotoxin derived from E. coli may be partly responsible for the increased susceptibility of C. parvum‐treated mice to E. coli infection.

    Original languageEnglish
    Pages (from-to)273-282
    Number of pages10
    JournalMICROBIOLOGY and IMMUNOLOGY
    Volume27
    Issue number3
    DOIs
    Publication statusPublished - Jan 1 1983

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    Propionibacterium acnes
    Escherichia coli Infections
    Lipopolysaccharides
    Peritoneal Macrophages
    Escherichia coli
    Infection
    Macrophages
    Bacteria
    Listeriosis
    Liver
    Lethal Dose 50
    Glucuronidase
    Hydrolases
    Hypersensitivity
    Spleen
    Acids

    All Science Journal Classification (ASJC) codes

    • Microbiology
    • Immunology
    • Virology

    Cite this

    Increased Susceptibility to Escherichia coli Infection in Mice Pretreated with Corynebacterium parvum. / Yoshikai, Yasunobu; Miake, Shunji; Sano, Masatoshi; Nomoto, Kikuo.

    In: MICROBIOLOGY and IMMUNOLOGY, Vol. 27, No. 3, 01.01.1983, p. 273-282.

    Research output: Contribution to journalArticle

    Yoshikai, Yasunobu ; Miake, Shunji ; Sano, Masatoshi ; Nomoto, Kikuo. / Increased Susceptibility to Escherichia coli Infection in Mice Pretreated with Corynebacterium parvum. In: MICROBIOLOGY and IMMUNOLOGY. 1983 ; Vol. 27, No. 3. pp. 273-282.
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