Increased susceptibility to primary infection with Listeria monocytogenes in germfree mice may be due to lack of accumulation of L- selectin+ CD44+ T cells in sites of inflammation

Hiroyuki Inagaki, Tatsuo Suzuki, Kikuo Nomoto, Yasunobu Yoshikai

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    57 Citations (Scopus)

    Abstract

    The host defense of germfree (GF) mice against primary infection with Listeria monocytogenes was compared with that of specific-pathogen-free (SPF) mice. In SPF mice, the numbers of bacteria in the peritoneal cavity, liver, and spleen decreased gradually to undetectable levels by day 8 after intraperitoneal infection with a sublethal dose (2 x 103 CFU) of L. monocytogenes. On the other hand, the elimination of bacteria in these organs of GF mice was significantly impaired at this stage after inoculation. We have reported previously that T cells coexpressing L- selectin and CD44 play an important role in protection against L. monocytogenes through trafficking to sites of inflammation. Consistent with nut previous findings, the number of unique L-selectin+ CD44+ T cells in the peritoneal cavity was remarkably increased on day 8 after infection in SPF mice, whereas such an increase was not evident in GF mice at this stage. Listeria-specific T-cell proliferation was normally detected in the lymph node cells of GF mice inoculated with L. monocytogenes, whereas the T-cell- proliferative response of the peritoneal exudate cells of GF mice was significantly impaired compared with that of SPF mice. These results suggest that the priming of T cells against listerial antigens normally occurs in the peripheral lymphoid organs of GF mice but the trafficking of the activated T cells to the inflamed sites may be severely impaired in GF mice, resulting in increased susceptibility to infection with L. monocytogenes.

    Original languageEnglish
    Pages (from-to)3280-3287
    Number of pages8
    JournalInfection and Immunity
    Volume64
    Issue number8
    Publication statusPublished - Aug 16 1996

    Fingerprint

    L-Selectin
    Listeria monocytogenes
    Inflammation
    T-Lymphocytes
    Infection
    Specific Pathogen-Free Organisms
    Peritoneal Cavity
    Bacteria
    Listeria
    Nuts
    Exudates and Transudates
    Spleen
    Lymph Nodes
    Cell Proliferation

    All Science Journal Classification (ASJC) codes

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

    Cite this

    Increased susceptibility to primary infection with Listeria monocytogenes in germfree mice may be due to lack of accumulation of L- selectin+ CD44+ T cells in sites of inflammation. / Inagaki, Hiroyuki; Suzuki, Tatsuo; Nomoto, Kikuo; Yoshikai, Yasunobu.

    In: Infection and Immunity, Vol. 64, No. 8, 16.08.1996, p. 3280-3287.

    Research output: Contribution to journalArticle

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    abstract = "The host defense of germfree (GF) mice against primary infection with Listeria monocytogenes was compared with that of specific-pathogen-free (SPF) mice. In SPF mice, the numbers of bacteria in the peritoneal cavity, liver, and spleen decreased gradually to undetectable levels by day 8 after intraperitoneal infection with a sublethal dose (2 x 103 CFU) of L. monocytogenes. On the other hand, the elimination of bacteria in these organs of GF mice was significantly impaired at this stage after inoculation. We have reported previously that T cells coexpressing L- selectin and CD44 play an important role in protection against L. monocytogenes through trafficking to sites of inflammation. Consistent with nut previous findings, the number of unique L-selectin+ CD44+ T cells in the peritoneal cavity was remarkably increased on day 8 after infection in SPF mice, whereas such an increase was not evident in GF mice at this stage. Listeria-specific T-cell proliferation was normally detected in the lymph node cells of GF mice inoculated with L. monocytogenes, whereas the T-cell- proliferative response of the peritoneal exudate cells of GF mice was significantly impaired compared with that of SPF mice. These results suggest that the priming of T cells against listerial antigens normally occurs in the peripheral lymphoid organs of GF mice but the trafficking of the activated T cells to the inflamed sites may be severely impaired in GF mice, resulting in increased susceptibility to infection with L. monocytogenes.",
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