Increased vitreous concentrations of MCP-1 and IL-6 after vitrectomy in patients with proliferative diabetic retinopathy: Possible association with postoperative macular oedema

Shigeo Yoshida, Yuki Kubo, Yoshiyuki Kobayashi, Yedi Zhou, Takahito Nakama, Muneo Yamaguchi, Takashi Tachibana, Keijiro Ishikawa, Ryoichi Arita, Shintaro Nakao, Yukio Sassa, Yuji Oshima, Toshihiro Kono, Tatsuro Ishibashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose To determine whether vitreal concentrations of MCP-1, IL-6 and IL-8 are altered after vitrectomy in patients with proliferative diabetic retinopathy (PDR) and to investigate whether the altered levels of these cytokines are associated with postoperative macular oedema. Methods Vitreous samples were collected from 36 eyes of 33 patients with PDR before pars plana vitrectomy without intraocular lens (IOL) implantation, and also from the same 36 eyes during IOL implantation surgery approximately 7 months after the initial vitrectomy. Levels of MCP-1, IL-6, IL-8 and vascular endothelial growth factor were measured by flow cytometry using cytometric bead array (CBA) technology. Results The mean vitreous levels of MCP-1, IL-6 and IL-8 in the samples collected before vitrectomy were significantly higher in patients with PDR than in control patients (p<0.0001). The levels of MCP-1 and IL-6 in the samples collected at the time of IOL implantation were significantly higher than those collected before vitrectomy ( p<0.05). In contrast, the level of IL-8 was significantly lower after vitrectomy ( p<0.05). The levels of IL-6 and IL-8, but not MCP-1, in the vitreous from eyes with PDR were inversely correlated with the interval between the initial vitrectomy and the time of implantation surgery. Among the vitrectomised patients, the mean vitreous level of MCP-1 in eyes with diabetic macular oedema (DME) was significantly higher than in those without DME ( p=0.028). Conclusions The elevated levels of MCP-1 and IL-6 may indicate prolonged inflammation even after successful vitrectomy, which can cause postoperative DME.

Original languageEnglish
Pages (from-to)960-966
Number of pages7
JournalBritish Journal of Ophthalmology
Volume99
Issue number7
DOIs
Publication statusPublished - Jul 1 2015

Fingerprint

Macular Edema
Vitrectomy
Diabetic Retinopathy
Interleukin-6
Interleukin-8
Intraocular Lens Implantation
Temazepam
Vascular Endothelial Growth Factor A
Flow Cytometry
Cytokines
Inflammation
Technology

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Increased vitreous concentrations of MCP-1 and IL-6 after vitrectomy in patients with proliferative diabetic retinopathy : Possible association with postoperative macular oedema. / Yoshida, Shigeo; Kubo, Yuki; Kobayashi, Yoshiyuki; Zhou, Yedi; Nakama, Takahito; Yamaguchi, Muneo; Tachibana, Takashi; Ishikawa, Keijiro; Arita, Ryoichi; Nakao, Shintaro; Sassa, Yukio; Oshima, Yuji; Kono, Toshihiro; Ishibashi, Tatsuro.

In: British Journal of Ophthalmology, Vol. 99, No. 7, 01.07.2015, p. 960-966.

Research output: Contribution to journalArticle

Yoshida, Shigeo ; Kubo, Yuki ; Kobayashi, Yoshiyuki ; Zhou, Yedi ; Nakama, Takahito ; Yamaguchi, Muneo ; Tachibana, Takashi ; Ishikawa, Keijiro ; Arita, Ryoichi ; Nakao, Shintaro ; Sassa, Yukio ; Oshima, Yuji ; Kono, Toshihiro ; Ishibashi, Tatsuro. / Increased vitreous concentrations of MCP-1 and IL-6 after vitrectomy in patients with proliferative diabetic retinopathy : Possible association with postoperative macular oedema. In: British Journal of Ophthalmology. 2015 ; Vol. 99, No. 7. pp. 960-966.
@article{bfe560dc046549cd87810dbc1f0166aa,
title = "Increased vitreous concentrations of MCP-1 and IL-6 after vitrectomy in patients with proliferative diabetic retinopathy: Possible association with postoperative macular oedema",
abstract = "Purpose To determine whether vitreal concentrations of MCP-1, IL-6 and IL-8 are altered after vitrectomy in patients with proliferative diabetic retinopathy (PDR) and to investigate whether the altered levels of these cytokines are associated with postoperative macular oedema. Methods Vitreous samples were collected from 36 eyes of 33 patients with PDR before pars plana vitrectomy without intraocular lens (IOL) implantation, and also from the same 36 eyes during IOL implantation surgery approximately 7 months after the initial vitrectomy. Levels of MCP-1, IL-6, IL-8 and vascular endothelial growth factor were measured by flow cytometry using cytometric bead array (CBA) technology. Results The mean vitreous levels of MCP-1, IL-6 and IL-8 in the samples collected before vitrectomy were significantly higher in patients with PDR than in control patients (p<0.0001). The levels of MCP-1 and IL-6 in the samples collected at the time of IOL implantation were significantly higher than those collected before vitrectomy ( p<0.05). In contrast, the level of IL-8 was significantly lower after vitrectomy ( p<0.05). The levels of IL-6 and IL-8, but not MCP-1, in the vitreous from eyes with PDR were inversely correlated with the interval between the initial vitrectomy and the time of implantation surgery. Among the vitrectomised patients, the mean vitreous level of MCP-1 in eyes with diabetic macular oedema (DME) was significantly higher than in those without DME ( p=0.028). Conclusions The elevated levels of MCP-1 and IL-6 may indicate prolonged inflammation even after successful vitrectomy, which can cause postoperative DME.",
author = "Shigeo Yoshida and Yuki Kubo and Yoshiyuki Kobayashi and Yedi Zhou and Takahito Nakama and Muneo Yamaguchi and Takashi Tachibana and Keijiro Ishikawa and Ryoichi Arita and Shintaro Nakao and Yukio Sassa and Yuji Oshima and Toshihiro Kono and Tatsuro Ishibashi",
year = "2015",
month = "7",
day = "1",
doi = "10.1136/bjophthalmol-2014-306366",
language = "English",
volume = "99",
pages = "960--966",
journal = "British Journal of Ophthalmology",
issn = "0007-1161",
publisher = "BMJ Publishing Group",
number = "7",

}

TY - JOUR

T1 - Increased vitreous concentrations of MCP-1 and IL-6 after vitrectomy in patients with proliferative diabetic retinopathy

T2 - Possible association with postoperative macular oedema

AU - Yoshida, Shigeo

AU - Kubo, Yuki

AU - Kobayashi, Yoshiyuki

AU - Zhou, Yedi

AU - Nakama, Takahito

AU - Yamaguchi, Muneo

AU - Tachibana, Takashi

AU - Ishikawa, Keijiro

AU - Arita, Ryoichi

AU - Nakao, Shintaro

AU - Sassa, Yukio

AU - Oshima, Yuji

AU - Kono, Toshihiro

AU - Ishibashi, Tatsuro

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Purpose To determine whether vitreal concentrations of MCP-1, IL-6 and IL-8 are altered after vitrectomy in patients with proliferative diabetic retinopathy (PDR) and to investigate whether the altered levels of these cytokines are associated with postoperative macular oedema. Methods Vitreous samples were collected from 36 eyes of 33 patients with PDR before pars plana vitrectomy without intraocular lens (IOL) implantation, and also from the same 36 eyes during IOL implantation surgery approximately 7 months after the initial vitrectomy. Levels of MCP-1, IL-6, IL-8 and vascular endothelial growth factor were measured by flow cytometry using cytometric bead array (CBA) technology. Results The mean vitreous levels of MCP-1, IL-6 and IL-8 in the samples collected before vitrectomy were significantly higher in patients with PDR than in control patients (p<0.0001). The levels of MCP-1 and IL-6 in the samples collected at the time of IOL implantation were significantly higher than those collected before vitrectomy ( p<0.05). In contrast, the level of IL-8 was significantly lower after vitrectomy ( p<0.05). The levels of IL-6 and IL-8, but not MCP-1, in the vitreous from eyes with PDR were inversely correlated with the interval between the initial vitrectomy and the time of implantation surgery. Among the vitrectomised patients, the mean vitreous level of MCP-1 in eyes with diabetic macular oedema (DME) was significantly higher than in those without DME ( p=0.028). Conclusions The elevated levels of MCP-1 and IL-6 may indicate prolonged inflammation even after successful vitrectomy, which can cause postoperative DME.

AB - Purpose To determine whether vitreal concentrations of MCP-1, IL-6 and IL-8 are altered after vitrectomy in patients with proliferative diabetic retinopathy (PDR) and to investigate whether the altered levels of these cytokines are associated with postoperative macular oedema. Methods Vitreous samples were collected from 36 eyes of 33 patients with PDR before pars plana vitrectomy without intraocular lens (IOL) implantation, and also from the same 36 eyes during IOL implantation surgery approximately 7 months after the initial vitrectomy. Levels of MCP-1, IL-6, IL-8 and vascular endothelial growth factor were measured by flow cytometry using cytometric bead array (CBA) technology. Results The mean vitreous levels of MCP-1, IL-6 and IL-8 in the samples collected before vitrectomy were significantly higher in patients with PDR than in control patients (p<0.0001). The levels of MCP-1 and IL-6 in the samples collected at the time of IOL implantation were significantly higher than those collected before vitrectomy ( p<0.05). In contrast, the level of IL-8 was significantly lower after vitrectomy ( p<0.05). The levels of IL-6 and IL-8, but not MCP-1, in the vitreous from eyes with PDR were inversely correlated with the interval between the initial vitrectomy and the time of implantation surgery. Among the vitrectomised patients, the mean vitreous level of MCP-1 in eyes with diabetic macular oedema (DME) was significantly higher than in those without DME ( p=0.028). Conclusions The elevated levels of MCP-1 and IL-6 may indicate prolonged inflammation even after successful vitrectomy, which can cause postoperative DME.

UR - http://www.scopus.com/inward/record.url?scp=84937733986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937733986&partnerID=8YFLogxK

U2 - 10.1136/bjophthalmol-2014-306366

DO - 10.1136/bjophthalmol-2014-306366

M3 - Article

C2 - 25631486

AN - SCOPUS:84937733986

VL - 99

SP - 960

EP - 966

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 7

ER -