Incubation type Si-based planar ion channel biosensor

Tsuneo Urisu, Toshifumi Asano, Zhenlong Zhang, Hidetaka Uno, Ryugo Tero, Han Junkyu, Isoda Hiroko, Yusuke Arima, Hiroo Iwata, Koji Shibasaki, Makoto Tominaga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (∼7 MΩ) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.

Original languageEnglish
Pages (from-to)2703-2709
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume391
Issue number8
DOIs
Publication statusPublished - Aug 1 2008

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Biosensing Techniques
Ion Channels
Biosensors
Seals
Transient Receptor Potential Channels
HEK293 Cells
Capsaicin
Sensors
Clamping devices
Signal-To-Noise Ratio
Silicon
Fibronectins
Cell culture
Signal to noise ratio
Cell Culture Techniques
Ligands
Coatings
Molecules
Substrates

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Urisu, T., Asano, T., Zhang, Z., Uno, H., Tero, R., Junkyu, H., ... Tominaga, M. (2008). Incubation type Si-based planar ion channel biosensor. Analytical and Bioanalytical Chemistry, 391(8), 2703-2709. https://doi.org/10.1007/s00216-008-1994-z

Incubation type Si-based planar ion channel biosensor. / Urisu, Tsuneo; Asano, Toshifumi; Zhang, Zhenlong; Uno, Hidetaka; Tero, Ryugo; Junkyu, Han; Hiroko, Isoda; Arima, Yusuke; Iwata, Hiroo; Shibasaki, Koji; Tominaga, Makoto.

In: Analytical and Bioanalytical Chemistry, Vol. 391, No. 8, 01.08.2008, p. 2703-2709.

Research output: Contribution to journalArticle

Urisu, T, Asano, T, Zhang, Z, Uno, H, Tero, R, Junkyu, H, Hiroko, I, Arima, Y, Iwata, H, Shibasaki, K & Tominaga, M 2008, 'Incubation type Si-based planar ion channel biosensor', Analytical and Bioanalytical Chemistry, vol. 391, no. 8, pp. 2703-2709. https://doi.org/10.1007/s00216-008-1994-z
Urisu T, Asano T, Zhang Z, Uno H, Tero R, Junkyu H et al. Incubation type Si-based planar ion channel biosensor. Analytical and Bioanalytical Chemistry. 2008 Aug 1;391(8):2703-2709. https://doi.org/10.1007/s00216-008-1994-z
Urisu, Tsuneo ; Asano, Toshifumi ; Zhang, Zhenlong ; Uno, Hidetaka ; Tero, Ryugo ; Junkyu, Han ; Hiroko, Isoda ; Arima, Yusuke ; Iwata, Hiroo ; Shibasaki, Koji ; Tominaga, Makoto. / Incubation type Si-based planar ion channel biosensor. In: Analytical and Bioanalytical Chemistry. 2008 ; Vol. 391, No. 8. pp. 2703-2709.
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