Incubation type Si-based planar ion channel biosensor

Tsuneo Urisu; Toshifumi Asano; Zhenlong Zhang; Hidetaka Uno; Ryugo Tero; Han Junkyu; Isoda Hiroko; Yusuke Arima; Hiroo Iwata; Koji Shibasaki; Makoto Tominaga

doi:10.1007/s00216-008-1994-z

Incubation type Si-based planar ion channel biosensor

Tsuneo Urisu, Toshifumi Asano, Zhenlong Zhang, Hidetaka Uno, Ryugo Tero, Han Junkyu, Isoda Hiroko, Yusuke Arima, Hiroo Iwata, Koji Shibasaki, Makoto Tominaga

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (∼7 MΩ) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.

Original language	English
Pages (from-to)	2703-2709
Number of pages	7
Journal	Analytical and Bioanalytical Chemistry
Volume	391
Issue number	8
DOIs	https://doi.org/10.1007/s00216-008-1994-z
Publication status	Published - Aug 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry

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10.1007/s00216-008-1994-z

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title = "Incubation type Si-based planar ion channel biosensor",

abstract = "A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (∼7 MΩ) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.",

author = "Tsuneo Urisu and Toshifumi Asano and Zhenlong Zhang and Hidetaka Uno and Ryugo Tero and Han Junkyu and Isoda Hiroko and Yusuke Arima and Hiroo Iwata and Koji Shibasaki and Makoto Tominaga",

note = "Funding Information: Acknowledgements This work was partly supported by NINS Cooperative Project Bio-molecular Sensor, and Grants-in-Aid for Scientific Research, Basic research (A) and Priority Area “Life Surveyor” promoted by the Ministry of Education, Culture, Sports, Science and Technology, Japan.",

year = "2008",

month = aug,

doi = "10.1007/s00216-008-1994-z",

language = "English",

volume = "391",

pages = "2703--2709",

journal = "Fresenius Zeitschrift fur Analytische Chemie",

issn = "0016-1152",

publisher = "Springer Verlag",

number = "8",

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T1 - Incubation type Si-based planar ion channel biosensor

AU - Urisu, Tsuneo

AU - Asano, Toshifumi

AU - Zhang, Zhenlong

AU - Uno, Hidetaka

AU - Tero, Ryugo

AU - Junkyu, Han

AU - Hiroko, Isoda

AU - Arima, Yusuke

AU - Iwata, Hiroo

AU - Shibasaki, Koji

AU - Tominaga, Makoto

N1 - Funding Information: Acknowledgements This work was partly supported by NINS Cooperative Project Bio-molecular Sensor, and Grants-in-Aid for Scientific Research, Basic research (A) and Priority Area “Life Surveyor” promoted by the Ministry of Education, Culture, Sports, Science and Technology, Japan.

PY - 2008/8

Y1 - 2008/8

N2 - A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (∼7 MΩ) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.

AB - A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (∼7 MΩ) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.

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DO - 10.1007/s00216-008-1994-z

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JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

IS - 8

ER -

Incubation type Si-based planar ion channel biosensor

Abstract

All Science Journal Classification (ASJC) codes

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