Independent evolution of a new allele of F1 pollen sterility gene S27 encoding mitochondrial ribosomal protein L27 in Oryza nivara

Khin Thanda Win, Yoshiyuki Yamagata, Yuta Miyazaki, Kazuyuki Doi, Hideshi Yasui, Atsushi Yoshimura

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Loss of function of duplicated genes plays an important role in the evolution of postzygotic reproductive isolation. The widespread occurrence of gene duplication followed by rapid loss of function of some of the duplicate gene copies suggests the independent evolution of loss-of-function alleles of duplicate genes in divergent lineages of speciation. Here, we found a novel loss-of-function allele of S27 in the Asian annual wild species Oryza nivara, designated S27-nivs, that leads to F1 pollen sterility in a cross between O. sativa and O. nivara. Genetic linkage analysis and complementation analysis demonstrated that S27-nivs lies at the same locus as the previously identiWed S27 locus and S27-nivs is a loss-of-function allele of S27. S27-nivs is composed of two tandem mitochondrial ribosomal protein L27 genes (mtRPL27a and mtRPL27b), both of which are inactive. The coding and promoter regions of S27-nivs showed a number of nucleotide diVerences from the functional S27-T65+ allele. The structure of S27-nivs is diVerent from that of a previously identiWed null S27 allele, S27-glums, in the South American wild rice species O. glumaepatula, in which mtRPL27a and mtRPL27b are absent. These results show that the mechanisms for loss-of-function of S27-nivs and S27-glums are diVerent. Our results provide experimental evidence that diVerent types of loss-of-function alleles are distributed in geographically and phylogenetically isolated species and represent a potential mechanism for postzygotic isolation in divergent species.

Original languageEnglish
Pages (from-to)385-394
Number of pages10
JournalTheoretical and Applied Genetics
Volume122
Issue number2
DOIs
Publication statusPublished - Feb 1 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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