Individual Variation and Age Dependency in the Radiosensitivity of Peripheral Blood T-lymphocytes from Normal Donors

Nan Mei, Hajime Imada, Satoshi Nomoto, Naoki Kunugita, Toshiyuki Norimura

Research output: Contribution to journalArticle

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Abstract

To investigate individual variation and age dependency in normal cell radiosensitivity, we measured the in vitro radiosensitivity of cultured peripheral blood T-lymphocytes derived from 56 healthy male blood donors. Dose-survival tests using colony formation assay were done with exponential growing T-cells (day 3, PHA-stimulated cells). 6-Thioguanine (6-TG)-resistant mutation assays at the hypoxanthine phosphoribosyl transferase (HPRT) locus were done with G0 phase T-cells (day 0, unstimulated cells). The mean inactivation dose (MID) computed by integration of the fitted survival curves was 1.25 ± 0.23 Gy (mean ± SD). The X-ray dose required to kill 90% of the cells (D10) was 2.81 ± 0.51 Gy. The MID ranged from 0.82 to 1.86 Gy with a coefficient of variation (CV) of 18%. The induced mutation frequencies (MF) per 106 cells at 2 Gy of X-rays ranged from 9.10 to 54.80 with a mean ± SD value of 24.63 ± 12.51 and a CV of 51%. It appears that the radiosensitivity of cell killing and mutagenicity varies among individuals. Although the spontaneous MF at the HPRT locus increases with age, the induced MF after exposure to 2 or 4 Gy of X-rays was not associated with age. Moreover, there were no significant correlations between age and MID values or the other dose-survival parameters. The findings indicate there is significant inter-individual variation in cellular radiosensitivity, but that in human T-lymphocytes aging does not affect the cytotoxic and mutagenic effects of X-irradiation.

Original languageEnglish
Pages (from-to)235-245
Number of pages11
JournalJournal of radiation research
Volume37
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

Fingerprint

Radiation Tolerance
lymphocytes
radiation tolerance
blood
Tissue Donors
mutations
T-Lymphocytes
dosage
Mutation Rate
deactivation
cells
Hypoxanthine
X-Rays
loci
Transferases
Survival
Thioguanine
Cell Cycle Resting Phase
x rays
coefficients

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Cite this

Individual Variation and Age Dependency in the Radiosensitivity of Peripheral Blood T-lymphocytes from Normal Donors. / Mei, Nan; Imada, Hajime; Nomoto, Satoshi; Kunugita, Naoki; Norimura, Toshiyuki.

In: Journal of radiation research, Vol. 37, No. 4, 01.01.1996, p. 235-245.

Research output: Contribution to journalArticle

Mei, Nan ; Imada, Hajime ; Nomoto, Satoshi ; Kunugita, Naoki ; Norimura, Toshiyuki. / Individual Variation and Age Dependency in the Radiosensitivity of Peripheral Blood T-lymphocytes from Normal Donors. In: Journal of radiation research. 1996 ; Vol. 37, No. 4. pp. 235-245.
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