TY - JOUR
T1 - Induced apoptosis in melanocytes cancer cell and oxidation in biomolecules through deuterium oxide generated from atmospheric pressure non-thermal plasma jet
AU - Kumar, Naresh
AU - Attri, Pankaj
AU - Yadav, Dharmendra Kumar
AU - Choi, Jinsung
AU - Choi, Eun Ha
AU - Uhm, Han Sup
N1 - Funding Information:
This work was financially supported by the SRC program (Grant # 2010-0029421) and by Basic Science Research Program of the National Research Foundation (NRF), and was also partially supported by CBDRC program in Defense Ministry. This work was also supported by R & D Program of ‘Plasma Advanced Technology for Agriculture and Food (Plasma Farming)’ through the National Fusion Research Institute of Korea (NFRI) funded by the Government funds and Kwangwoon University 2014.
PY - 2014/12/23
Y1 - 2014/12/23
N2 - Recently, atmospheric-pressure non-thermal plasma-jets (APPJ) are being for the cancer treatment. However, APPJ still has drawbacks such as efficiency and rise in temperature after treatment. So, in this work, a synergetic agent D2O vapour is attached to APPJ which not only increase the efficiency of plasma source against cancer treatment, but also controlled the temperature during the treatment.OD generated by the combination of D2O + N2 plasma helped in enhancing the efficiency of APPJ. We observed ODinduced apoptosis on melanocytes G361 cancer cells through DNA damage signalling cascade. Additionally, we observed that plasma induces ROS, which activated MAPK p38 and inhibits p42/p44 MAPK, leading to cancer cell death. We have also studied DNA oxidation by extracting DNA from treated cancer cell and then analysed the effects of OD/OH/D2O2/H2O2 on protein modification and oxidation. Additionally, we attempted molecular docking approaches to check the action of D2O2 on the apoptosis related genes. Further, we confirmed the formation of OD/OH simultaneously in the solution using optical emission spectroscopy. Moreover, the simultaneous generation of D2O2/H2O2 was detected by the use of confocal Raman spectroscopy and density measurements.
AB - Recently, atmospheric-pressure non-thermal plasma-jets (APPJ) are being for the cancer treatment. However, APPJ still has drawbacks such as efficiency and rise in temperature after treatment. So, in this work, a synergetic agent D2O vapour is attached to APPJ which not only increase the efficiency of plasma source against cancer treatment, but also controlled the temperature during the treatment.OD generated by the combination of D2O + N2 plasma helped in enhancing the efficiency of APPJ. We observed ODinduced apoptosis on melanocytes G361 cancer cells through DNA damage signalling cascade. Additionally, we observed that plasma induces ROS, which activated MAPK p38 and inhibits p42/p44 MAPK, leading to cancer cell death. We have also studied DNA oxidation by extracting DNA from treated cancer cell and then analysed the effects of OD/OH/D2O2/H2O2 on protein modification and oxidation. Additionally, we attempted molecular docking approaches to check the action of D2O2 on the apoptosis related genes. Further, we confirmed the formation of OD/OH simultaneously in the solution using optical emission spectroscopy. Moreover, the simultaneous generation of D2O2/H2O2 was detected by the use of confocal Raman spectroscopy and density measurements.
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U2 - 10.1038/srep07589
DO - 10.1038/srep07589
M3 - Article
C2 - 25534001
AN - SCOPUS:84930641560
VL - 4
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 7589
ER -
