Induced monoterpene and lignin production in mechanically stressed and fungal elicited cultured Cupressus lusitanica cells

Ransika De Alwis, Koki Fujita, Tatsuya Ashitani, Ken'Ichi Kuroda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cultured Cupressus lusitanica cells induced by various stresses are thought to produce different complexes of defense chemicals to optimize defense. To compare the induced products of two stimulations, we investigated the emission of monoterpenes, biosynthesis of β-thujaplicin, and accumulation of lignin in mechanically stressed and fungal elicited cultured C. lusitanica cells. Both mechanical stress and fungal elicitor caused emission of qualitatively similar monoterpene blends indicating de novo biosynthesis of these compounds after stimulation, while mechanical stress alone is sufficient to induce fungal elicitor-related monoterpene emission. Sabinene and limonene were the dominant compounds over the time course in both volatile blends. Although the emitted volatile blends were qualitatively similar, the time course and the relative ratios of the constituents of the volatile blends differed with the type of stimulation. While fungal elicited cells produced significant amounts of β-thujaplicin over the 5-day time course, no β-thujaplicin was observed in the mechanically stressed cells. The production of β-thujaplicin was the main dissimilarity of the induced products of these two treatments, suggesting that synthesis of β-thujaplicin is not a general response to all types of stresses, but is a specific response and serves as a strong toxic compound against already invaded fungus. Significantly higher amounts of lignin accumulations were observed in the fungal elicited and mechanically stressed cells on the 5th day after induction. Based on these results, we suggest the composition of induced products was dependent on the method of stimulation.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalPlant Biotechnology Reports
Volume3
Issue number1
DOIs
Publication statusPublished - Feb 1 2009

Fingerprint

Cupressus lusitanica
Cupressus
Monoterpenes
Lignin
monoterpenoids
lignin
Mechanical Stress
mechanical stress
cells
biosynthesis
sabinene
Poisons
toxic substances
Fungi
fungi
synthesis

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Biotechnology

Cite this

Induced monoterpene and lignin production in mechanically stressed and fungal elicited cultured Cupressus lusitanica cells. / De Alwis, Ransika; Fujita, Koki; Ashitani, Tatsuya; Kuroda, Ken'Ichi.

In: Plant Biotechnology Reports, Vol. 3, No. 1, 01.02.2009, p. 57-65.

Research output: Contribution to journalArticle

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