Inducible production of erythropoietin using new runaway-type vector by mammalian cell culture in spinner flask

Hideyo Kirinaka, Masamichi Kamihira, Takeshi Kobayashi, Shinji Iijima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human erythropoietin (HuEPO) gene was inserted into an SV40-based episomal vector with a temperature-sensitive large T antigen which enabled uncontrolled plasmid replication and inducible HuEPO production at temperatures below 33°C. Good productivity of HuEPO was obtained when temperature was lowered to 30°C at relatively higher cell density in Petri dish culture. Therefore, we cultured the cells immobilized on microcarriers in a spinner flask at 37°C until the cell density reached 1.8 × 104 cells/cm2-microcarrier surface area, and then the temperature was lowered to 30°C for HuEPO production. Using this process, HuEPO productivity was enhanced 7-18 times that of the Petri dish cultures, and a high concentration of HuEPO was accumulated in the medium.

Original languageEnglish
Pages (from-to)483-485
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume78
Issue number6
DOIs
Publication statusPublished - 1994
Externally publishedYes

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Homeless Youth
Erythropoietin
Cell culture
Cell Culture Techniques
Temperature
Productivity
Antigens
Cell Count
Genes
Viral Tumor Antigens
Cultured Cells
Plasmids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Inducible production of erythropoietin using new runaway-type vector by mammalian cell culture in spinner flask. / Kirinaka, Hideyo; Kamihira, Masamichi; Kobayashi, Takeshi; Iijima, Shinji.

In: Journal of Fermentation and Bioengineering, Vol. 78, No. 6, 1994, p. 483-485.

Research output: Contribution to journalArticle

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