Induction and purification of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae grown in ovalbumin

K. Takegawa, M. Nakoshi, S. Iwahara, K. Yamamoto, T. Tochikura

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Arthrobacter protophormiae produced a high level of extracellular endo-β-N-acetylglucosaminidase when cells were grown in a medium containing ovalbumin. The enzyme was incuded by the glycopeptide fraction of ovalbumin prepared by pronase digestion. Production of the enzyme was also induced by glycoproteins such as yeast invertase and bovine ribonuclease B but not by monosaccharides such as mannose, N-acetylglucosamine, and galactose. The enzyme was purified to homogeneity as demonstrated by polyacrylamide gel electrophoresis and has an apparent molecular weight of about 80,000. The enzyme showed a broad optimum pH in the range of pH 5.0 to 11.0. The enzyme hydrolyzed all heterogeneous ovalbumin glycopeptides, although the hydrolysis rates for hybrid type glycopeptides were very low. The substrate specificity of A. protophormiae endo-β-N-acetylglucosaminidase was very similar to that of Endo-C(II) from Clostridium perfringens. Therefore, the enzyme induction by A. protophormiae seems to have a close relation to the substrate specificity of the enzyme.

Original languageEnglish
Pages (from-to)3107-3112
Number of pages6
JournalApplied and environmental microbiology
Volume55
Issue number12
Publication statusPublished - Dec 21 1989

Fingerprint

Arthrobacter protophormiae
Arthrobacter
Acetylglucosaminidase
ovalbumin
Ovalbumin
purification
enzyme
glycopeptides
Glycopeptides
Enzymes
enzymes
substrate specificity
Substrate Specificity
beta-Fructofuranosidase
N-acetylglucosamine
Pronase
Clostridium perfringens
Enzyme Induction
Acetylglucosamine
ribonucleases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Induction and purification of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae grown in ovalbumin. / Takegawa, K.; Nakoshi, M.; Iwahara, S.; Yamamoto, K.; Tochikura, T.

In: Applied and environmental microbiology, Vol. 55, No. 12, 21.12.1989, p. 3107-3112.

Research output: Contribution to journalArticle

Takegawa, K. ; Nakoshi, M. ; Iwahara, S. ; Yamamoto, K. ; Tochikura, T. / Induction and purification of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae grown in ovalbumin. In: Applied and environmental microbiology. 1989 ; Vol. 55, No. 12. pp. 3107-3112.
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