Induction and suppression of contact sensitivity to fluorescein isothiocyanate (FITC)

Masutaka Furue, K. Tamaki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although both fluorescein isothiocyanate (FITC) and trinitrophenyl (TNP) covalently couple primarily to the ε-amino group of lysine residues of surface glycoprotein, FITC is structurally different from TNP. In this paper, we investigated the immune regulatory mechanisms in contact sensitivity (CS) to FITC by the administration of FITC and FITC-conjugated epidermal cells (FITC-EC) via various routes. Mice injected with FITC via i.p. or i.v. route did not induce CS but induced hyporesponsiveness to the following sensitization with FITC painting. Mice injected with FITC via s.c. route induced neither CS nor hyporesponsiveness to the following FITC painting. Administration of FITC via i.v. route was demonstrated to induce hapten-specific suppressor T cells. Inoculation of FITC-EC via s.c. or i.p. route induced CS, whereas injection of FITC-EC via i.v. route did not induce CS but induced hyporesponsiveness to the following FITC painting. The results are compared with the previous data obtained by trinitrobenzene sulfonate and trinitrophenyl-conjugated epidermal cells.

Original languageEnglish
Pages (from-to)139-142
Number of pages4
JournalJournal of Investigative Dermatology
Volume85
Issue number2
DOIs
Publication statusPublished - Jan 1 1985
Externally publishedYes

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Contact Dermatitis
Fluorescein
Paintings
Painting
isothiocyanic acid
Trinitrobenzenesulfonic Acid
T-cells
Haptens
Membrane Glycoproteins
Lysine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Induction and suppression of contact sensitivity to fluorescein isothiocyanate (FITC). / Furue, Masutaka; Tamaki, K.

In: Journal of Investigative Dermatology, Vol. 85, No. 2, 01.01.1985, p. 139-142.

Research output: Contribution to journalArticle

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