We have previously shown that a 54 kDa protein in rat liver is highly homologous to selenium-binding protein (SeBP) or acetaminophen-binding protein (APBP) in mice and is highly inducible by treatment with 3,3',4,4',5-pentachlorobiphenyl or 3-methylcholanthrene. In this study, we examine the effect of six typical inducers, 3-methylcholanthrene (MC), isosafrole (ISO), phenobarbital (PB), dexamethasone (DEX), clofibrate (CLO), pyrazole (PYR) and butylated hydroxytoluene (BHT), on the expression level of this 54 kDa protein. Male Wistar rats were given each inducer following a predetermined schedule. Among these inducers, the 54 kDa protein was inducible by MC and BHT. The response to MC and BHT was compared with that of NAD(P)H: quinone oxidoreductase and ethoxyresorufin O-deethylase activities. The induction mechanisms and physiological role of the 54 kDa protein are discussed in the light of our results. Copyright (C) 1998 Elsevier Science B.V.
All Science Journal Classification (ASJC) codes
- Health, Toxicology and Mutagenesis