Induction of apoptosis by low-molecular-weight fucoidan through calcium- and caspase-dependent mitochondrial pathways in MDA-MB-231 breast cancer cells

Zhongyuan Zhang, Kiichiro Teruya, Hiroshi Eto, Sanetaka Shirahata

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Fucoidan, a fucose-rich polysaccharide extracted from brown seaweed, has antitumor, anticoagulant, antiviral, anti-inflammatory, and antibacterial activities. Several studies have shown that a fucoidan treatment of cancer cells induced cytotoxicity and apoptosis and inhibited angiogenesis and invasion. We investigated in the present study the effect of low-molecular-weight fucoidan (LMWF) on apoptosis in estrogen receptornegative MDA-MB-231 human breast cancer cells. The LMWF treatment of MDA-MB-231 cells was associated with the activation of caspases and mitochondrial dysfunction, including dissipation of the mitochondrial membrane potential (ΔΨm), alteration of Ca2+ homeostasis, cytochrome c release, and decreased expression of antiapoptotic Bcl-2 family proteins. Understanding the molecular events that mediated LMWF-induced MDAMB-231 cell death will contribute to a more rational approach to cancer chemotherapy.

Original languageEnglish
Pages (from-to)235-242
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number2
DOIs
Publication statusPublished - Apr 2 2013

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Caspases
Molecular Weight
Molecular weight
Cells
Apoptosis
Breast Neoplasms
Calcium
Seaweed
Chemotherapy
Fucose
Mitochondrial Membrane Potential
Cell death
Cytotoxicity
Cytochromes c
Anticoagulants
Antiviral Agents
Polysaccharides
Neoplasms
Estrogens
Homeostasis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Induction of apoptosis by low-molecular-weight fucoidan through calcium- and caspase-dependent mitochondrial pathways in MDA-MB-231 breast cancer cells. / Zhang, Zhongyuan; Teruya, Kiichiro; Eto, Hiroshi; Shirahata, Sanetaka.

In: Bioscience, Biotechnology and Biochemistry, Vol. 77, No. 2, 02.04.2013, p. 235-242.

Research output: Contribution to journalArticle

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