Induction of bone repair in rat calvarial defects using a combination of hydroxyapatite with phosphatidylserine liposomes

Junko Hatakeyama, Hisashi Anan, Yuji Hatakeyama, Noriyoshi Matsumoto, Fumiko Takayama, Zhou Wu, Etsuko Matsuzaki, Masahiko Minakami, Toshio Izumi, Hiroshi Nakanishi

Research output: Contribution to journalArticle

Abstract

Phosphatidylserine (PS)―normally present on the inner leaflet of the plasma membrane― translocates to the outer leaflet at an early stage of apoptosis. PS-containing liposomes (PSLs) can mimic the effect of apoptotic cells in inducing the secretion of prostaglandin E2 from phagocytes and inhibiting the maturation of dendritic cells and osteoclast precursors. The present study attempted to evaluate the effect of calcium phosphate (in the form of hydroxyapatite [HAP]) in the presence or absence of PSLs for repair of rat calvarial bone defects. The defects, each 5 mm in diameter, were created in the calvaria parietal bone of 8-week-old Wistar rats and subjected to one of the following treatments: no augmentation (Sham), HAP alone, or a mixture of HAP and PSL (HAP+PSL). Micro-computed tomography data showed that the HAP+PSL complexes promoted greater bone regeneration in comparison with either the Sham procedure or HAP alone at 4 and 8 weeks after implantation. The regeneration of calvarial bone defects induced by PSLs was mediated partly through upregulation of the osteogenic marker Alkaline Phosphatase, Type I collagen, osteocalcin, Runx2, and Osterix mRNAs. These data are the first to show that PSLs can influence bone regeneration by regulating osteoblast differentiation.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalJournal of Oral Science
Volume61
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

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Phosphatidylserines
Durapatite
Liposomes
Bone and Bones
Bone Regeneration
Parietal Bone
Osteocalcin
Osteoclasts
Phagocytes
Collagen Type I
Osteoblasts
Dinoprostone
Skull
Dendritic Cells
Alkaline Phosphatase
Wistar Rats
Up-Regulation
Tomography
Cell Membrane
Apoptosis

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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Induction of bone repair in rat calvarial defects using a combination of hydroxyapatite with phosphatidylserine liposomes. / Hatakeyama, Junko; Anan, Hisashi; Hatakeyama, Yuji; Matsumoto, Noriyoshi; Takayama, Fumiko; Wu, Zhou; Matsuzaki, Etsuko; Minakami, Masahiko; Izumi, Toshio; Nakanishi, Hiroshi.

In: Journal of Oral Science, Vol. 61, No. 1, 01.01.2019, p. 111-118.

Research output: Contribution to journalArticle

Hatakeyama, J, Anan, H, Hatakeyama, Y, Matsumoto, N, Takayama, F, Wu, Z, Matsuzaki, E, Minakami, M, Izumi, T & Nakanishi, H 2019, 'Induction of bone repair in rat calvarial defects using a combination of hydroxyapatite with phosphatidylserine liposomes', Journal of Oral Science, vol. 61, no. 1, pp. 111-118. https://doi.org/10.2334/josnusd.17-0488
Hatakeyama, Junko ; Anan, Hisashi ; Hatakeyama, Yuji ; Matsumoto, Noriyoshi ; Takayama, Fumiko ; Wu, Zhou ; Matsuzaki, Etsuko ; Minakami, Masahiko ; Izumi, Toshio ; Nakanishi, Hiroshi. / Induction of bone repair in rat calvarial defects using a combination of hydroxyapatite with phosphatidylserine liposomes. In: Journal of Oral Science. 2019 ; Vol. 61, No. 1. pp. 111-118.
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