Abstract
Phosphatidylserine (PS)―normally present on the inner leaflet of the plasma membrane― translocates to the outer leaflet at an early stage of apoptosis. PS-containing liposomes (PSLs) can mimic the effect of apoptotic cells in inducing the secretion of prostaglandin E2 from phagocytes and inhibiting the maturation of dendritic cells and osteoclast precursors. The present study attempted to evaluate the effect of calcium phosphate (in the form of hydroxyapatite [HAP]) in the presence or absence of PSLs for repair of rat calvarial bone defects. The defects, each 5 mm in diameter, were created in the calvaria parietal bone of 8-week-old Wistar rats and subjected to one of the following treatments: no augmentation (Sham), HAP alone, or a mixture of HAP and PSL (HAP+PSL). Micro-computed tomography data showed that the HAP+PSL complexes promoted greater bone regeneration in comparison with either the Sham procedure or HAP alone at 4 and 8 weeks after implantation. The regeneration of calvarial bone defects induced by PSLs was mediated partly through upregulation of the osteogenic marker Alkaline Phosphatase, Type I collagen, osteocalcin, Runx2, and Osterix mRNAs. These data are the first to show that PSLs can influence bone regeneration by regulating osteoblast differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 111-118 |
| Number of pages | 8 |
| Journal | Journal of Oral Science |
| Volume | 61 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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All Science Journal Classification (ASJC) codes
- Dentistry(all)
Cite this
Induction of bone repair in rat calvarial defects using a combination of hydroxyapatite with phosphatidylserine liposomes. / Hatakeyama, Junko; Anan, Hisashi; Hatakeyama, Yuji; Matsumoto, Noriyoshi; Takayama, Fumiko; Wu, Zhou; Matsuzaki, Etsuko; Minakami, Masahiko; Izumi, Toshio; Nakanishi, Hiroshi.
In: Journal of Oral Science, Vol. 61, No. 1, 01.01.2019, p. 111-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of bone repair in rat calvarial defects using a combination of hydroxyapatite with phosphatidylserine liposomes
AU - Hatakeyama, Junko
AU - Anan, Hisashi
AU - Hatakeyama, Yuji
AU - Matsumoto, Noriyoshi
AU - Takayama, Fumiko
AU - Wu, Zhou
AU - Matsuzaki, Etsuko
AU - Minakami, Masahiko
AU - Izumi, Toshio
AU - Nakanishi, Hiroshi
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Phosphatidylserine (PS)―normally present on the inner leaflet of the plasma membrane― translocates to the outer leaflet at an early stage of apoptosis. PS-containing liposomes (PSLs) can mimic the effect of apoptotic cells in inducing the secretion of prostaglandin E2 from phagocytes and inhibiting the maturation of dendritic cells and osteoclast precursors. The present study attempted to evaluate the effect of calcium phosphate (in the form of hydroxyapatite [HAP]) in the presence or absence of PSLs for repair of rat calvarial bone defects. The defects, each 5 mm in diameter, were created in the calvaria parietal bone of 8-week-old Wistar rats and subjected to one of the following treatments: no augmentation (Sham), HAP alone, or a mixture of HAP and PSL (HAP+PSL). Micro-computed tomography data showed that the HAP+PSL complexes promoted greater bone regeneration in comparison with either the Sham procedure or HAP alone at 4 and 8 weeks after implantation. The regeneration of calvarial bone defects induced by PSLs was mediated partly through upregulation of the osteogenic marker Alkaline Phosphatase, Type I collagen, osteocalcin, Runx2, and Osterix mRNAs. These data are the first to show that PSLs can influence bone regeneration by regulating osteoblast differentiation.
AB - Phosphatidylserine (PS)―normally present on the inner leaflet of the plasma membrane― translocates to the outer leaflet at an early stage of apoptosis. PS-containing liposomes (PSLs) can mimic the effect of apoptotic cells in inducing the secretion of prostaglandin E2 from phagocytes and inhibiting the maturation of dendritic cells and osteoclast precursors. The present study attempted to evaluate the effect of calcium phosphate (in the form of hydroxyapatite [HAP]) in the presence or absence of PSLs for repair of rat calvarial bone defects. The defects, each 5 mm in diameter, were created in the calvaria parietal bone of 8-week-old Wistar rats and subjected to one of the following treatments: no augmentation (Sham), HAP alone, or a mixture of HAP and PSL (HAP+PSL). Micro-computed tomography data showed that the HAP+PSL complexes promoted greater bone regeneration in comparison with either the Sham procedure or HAP alone at 4 and 8 weeks after implantation. The regeneration of calvarial bone defects induced by PSLs was mediated partly through upregulation of the osteogenic marker Alkaline Phosphatase, Type I collagen, osteocalcin, Runx2, and Osterix mRNAs. These data are the first to show that PSLs can influence bone regeneration by regulating osteoblast differentiation.
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UR - http://www.scopus.com/inward/citedby.url?scp=85063953422&partnerID=8YFLogxK
U2 - 10.2334/josnusd.17-0488
DO - 10.2334/josnusd.17-0488
M3 - Article
C2 - 30918207
AN - SCOPUS:85063953422
VL - 61
SP - 111
EP - 118
JO - Journal of oral science
JF - Journal of oral science
SN - 1343-4934
IS - 1
ER -