Abstract
Background: This study was designed to assess the therapeutic effect of urokinase-targeted recombinant oncolytic Sendai virus, termed “BioKnife,” on anaplastic thyroid carcinoma (ATC). Methods: Urokinase activity was investigated in human ATC cell lines, and BioKnife cytotoxicity against the cell lines was evaluated in vitro. Orthotopic mouse models of ATC were treated with three intratumoral injections of BioKnife, control virus, or phosphate-buffered saline (PBS) and were observed daily until >20% weight loss occurred. Results: All three ATC cell lines showed a high level of urokinase activity. BioKnife induced urokinase-dependent cell fusion and cytotoxicity in all cell lines. Orthotopic models treated with BioKnife showed significantly prolonged survival compared with models treated with control virus or PBS (BioKnife 41.6 ± 15.0, control virus 17.0 ± 2.9, PBS 17.7 ± 6.3 days). Conclusions: BioKnife exerted therapeutic effects in orthotopic ATC mouse models. Thus, BioKnife represents a possible treatment option for ATC.
| Original language | English |
|---|---|
| Pages (from-to) | 2873-2882 |
| Number of pages | 10 |
| Journal | Head and Neck |
| Volume | 41 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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All Science Journal Classification (ASJC) codes
- Otorhinolaryngology
Cite this
Induction of cell fusion/apoptosis in anaplastic thyroid carcinoma in orthotopic mouse model by urokinase-specific oncolytic Sendai virus. / Miyagawa, Yoshihiro; Araki, Koji; Yamashita, Taku; Tanaka, Shingo; Tanaka, Yuya; Tomifuji, Masayuki; Ueda, Yasuji; Yonemitsu, Yoshikazu; Shimada, Hideaki; Shiotani, Akihiro.
In: Head and Neck, Vol. 41, No. 9, 01.01.2019, p. 2873-2882.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of cell fusion/apoptosis in anaplastic thyroid carcinoma in orthotopic mouse model by urokinase-specific oncolytic Sendai virus
AU - Miyagawa, Yoshihiro
AU - Araki, Koji
AU - Yamashita, Taku
AU - Tanaka, Shingo
AU - Tanaka, Yuya
AU - Tomifuji, Masayuki
AU - Ueda, Yasuji
AU - Yonemitsu, Yoshikazu
AU - Shimada, Hideaki
AU - Shiotani, Akihiro
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background: This study was designed to assess the therapeutic effect of urokinase-targeted recombinant oncolytic Sendai virus, termed “BioKnife,” on anaplastic thyroid carcinoma (ATC). Methods: Urokinase activity was investigated in human ATC cell lines, and BioKnife cytotoxicity against the cell lines was evaluated in vitro. Orthotopic mouse models of ATC were treated with three intratumoral injections of BioKnife, control virus, or phosphate-buffered saline (PBS) and were observed daily until >20% weight loss occurred. Results: All three ATC cell lines showed a high level of urokinase activity. BioKnife induced urokinase-dependent cell fusion and cytotoxicity in all cell lines. Orthotopic models treated with BioKnife showed significantly prolonged survival compared with models treated with control virus or PBS (BioKnife 41.6 ± 15.0, control virus 17.0 ± 2.9, PBS 17.7 ± 6.3 days). Conclusions: BioKnife exerted therapeutic effects in orthotopic ATC mouse models. Thus, BioKnife represents a possible treatment option for ATC.
AB - Background: This study was designed to assess the therapeutic effect of urokinase-targeted recombinant oncolytic Sendai virus, termed “BioKnife,” on anaplastic thyroid carcinoma (ATC). Methods: Urokinase activity was investigated in human ATC cell lines, and BioKnife cytotoxicity against the cell lines was evaluated in vitro. Orthotopic mouse models of ATC were treated with three intratumoral injections of BioKnife, control virus, or phosphate-buffered saline (PBS) and were observed daily until >20% weight loss occurred. Results: All three ATC cell lines showed a high level of urokinase activity. BioKnife induced urokinase-dependent cell fusion and cytotoxicity in all cell lines. Orthotopic models treated with BioKnife showed significantly prolonged survival compared with models treated with control virus or PBS (BioKnife 41.6 ± 15.0, control virus 17.0 ± 2.9, PBS 17.7 ± 6.3 days). Conclusions: BioKnife exerted therapeutic effects in orthotopic ATC mouse models. Thus, BioKnife represents a possible treatment option for ATC.
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U2 - 10.1002/hed.25769
DO - 10.1002/hed.25769
M3 - Article
VL - 41
SP - 2873
EP - 2882
JO - Head and Neck Surgery
JF - Head and Neck Surgery
SN - 1043-3074
IS - 9
ER -