Induction of epithelial-mesenchymal transition-related genes by benzo[a]pyrene in lung cancer cells

Ichiro Yoshino, Takuro Kometani, Fumihiro Shoji, Atsushi Osoegawa, Taro Ohba, Hidenori Kouso, Tomoyoshi Takenaka, Tomofumi Yohena, Yoshihiko Maehara

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Abstract

BACKGROUND. It is believed that epithelial-mesenchymal transition (EMT) occurs during the development and progression of cancer; however, the correlation between tobacco smoking and EMT remains to be elucidated. METHODS. Cells from the bronchioloalveolar carcinoma cell line A549 were exposed to benzo(a)pyrene (B[a]P) for 24 weeks, and morphology, proliferative activity, and gene expression profiles were analyzed. RESULTS. Although no apparent morphologic changes were observed, the B[a]P-exposed A549 cells exhibited enhanced proliferative activity in 1% bovine serum that contained medium, and dramatic changes in expression levels were observed in a large number of genes. Of those, the expression of EMT-related genes, such as migration-stimulating factor, plasminogen activator inhibitor-1, fibronectin, twist, transforming growth factor-β2, basic fibroblast growth factor, and electron transport system, were up-regulated; whereas gene expression of E-cadherin was decreased. Most enhanced expression levels remained 8 weeks after the retrieval of B[a]P in culture. CONCLUSIONS. The current results indicated that B[a]P seems to induce EMT in lung cancer cells, and it also may drive disease progression in patients with lung cancer.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalCancer
Volume110
Issue number2
DOIs
Publication statusPublished - Jul 15 2007

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Epithelial-Mesenchymal Transition
Benzo(a)pyrene
Lung Neoplasms
Genes
Bronchiolo-Alveolar Adenocarcinoma
Plasminogen Activator Inhibitor 1
Transforming Growth Factors
Fibroblast Growth Factor 2
Cadherins
Electron Transport
Transcriptome
Fibronectins
Disease Progression
Smoking
Gene Expression
Serum
Neoplasms
A549 Cells

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Induction of epithelial-mesenchymal transition-related genes by benzo[a]pyrene in lung cancer cells. / Yoshino, Ichiro; Kometani, Takuro; Shoji, Fumihiro; Osoegawa, Atsushi; Ohba, Taro; Kouso, Hidenori; Takenaka, Tomoyoshi; Yohena, Tomofumi; Maehara, Yoshihiko.

In: Cancer, Vol. 110, No. 2, 15.07.2007, p. 369-374.

Research output: Contribution to journalArticle

Yoshino, I, Kometani, T, Shoji, F, Osoegawa, A, Ohba, T, Kouso, H, Takenaka, T, Yohena, T & Maehara, Y 2007, 'Induction of epithelial-mesenchymal transition-related genes by benzo[a]pyrene in lung cancer cells', Cancer, vol. 110, no. 2, pp. 369-374. https://doi.org/10.1002/cncr.22728
Yoshino, Ichiro ; Kometani, Takuro ; Shoji, Fumihiro ; Osoegawa, Atsushi ; Ohba, Taro ; Kouso, Hidenori ; Takenaka, Tomoyoshi ; Yohena, Tomofumi ; Maehara, Yoshihiko. / Induction of epithelial-mesenchymal transition-related genes by benzo[a]pyrene in lung cancer cells. In: Cancer. 2007 ; Vol. 110, No. 2. pp. 369-374.
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