Induction of germline transcription in the TCRγ, locus by Stat5: Implications for accessibility control by the IL-7 receptor

Sang Kyu Ye, Kazushige Maki, Toshio Kitamura, Shinji Sunaga, Koichi Akashi, Jos Domen, Irving L. Weissman, Tasuku Honjo, Koichi Ikuta

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

IL-7 receptor (IL-7R) plays critical roles in lymphocyte development by promoting survival and proliferation and by inducing V(D)J recombination in TCR and Ig loci. Here, we demonstrate that IL-7R-activated Stat5 binds to consensus motifs in the 5' regions of Jγ segments and induces germline transcripts. We also show that a constitutively active form of Stat5 restores V-J recombination of TCRγ genes and partially rescues T cell development from IL-7R(-/-) T cell precursors, especially in favor of γδ T cells. Therefore, this study reveals a potential role of Stat5 in T cell development and also implies that IL-7R may control the accessibility of the TCRγ, locus through Stat5-induced germline transcription.

Original languageEnglish
Pages (from-to)213-223
Number of pages11
JournalImmunity
Volume11
Issue number2
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Interleukin-7 Receptors
T-Lymphocytes
V(D)J Recombination
T-Lymphoid Precursor Cells
Genetic Recombination
Lymphocytes
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Induction of germline transcription in the TCRγ, locus by Stat5 : Implications for accessibility control by the IL-7 receptor. / Ye, Sang Kyu; Maki, Kazushige; Kitamura, Toshio; Sunaga, Shinji; Akashi, Koichi; Domen, Jos; Weissman, Irving L.; Honjo, Tasuku; Ikuta, Koichi.

In: Immunity, Vol. 11, No. 2, 01.01.1999, p. 213-223.

Research output: Contribution to journalArticle

Ye, SK, Maki, K, Kitamura, T, Sunaga, S, Akashi, K, Domen, J, Weissman, IL, Honjo, T & Ikuta, K 1999, 'Induction of germline transcription in the TCRγ, locus by Stat5: Implications for accessibility control by the IL-7 receptor', Immunity, vol. 11, no. 2, pp. 213-223. https://doi.org/10.1016/S1074-7613(00)80096-5
Ye, Sang Kyu ; Maki, Kazushige ; Kitamura, Toshio ; Sunaga, Shinji ; Akashi, Koichi ; Domen, Jos ; Weissman, Irving L. ; Honjo, Tasuku ; Ikuta, Koichi. / Induction of germline transcription in the TCRγ, locus by Stat5 : Implications for accessibility control by the IL-7 receptor. In: Immunity. 1999 ; Vol. 11, No. 2. pp. 213-223.
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AU - Sunaga, Shinji

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AU - Domen, Jos

AU - Weissman, Irving L.

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