Induction of granulocyte colony-stimulating factor by globular adiponectin via the MEK-ERK pathway

Noriaki Kamio, Sumio Akifusa, Noboru Yamaguchi, Yoshihisa Yamashita

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Adiponectin, an adipocyte-derived cytokine, affects a number of physiological processes, including immune function and inflammation. We investigated whether globular adiponectin (gAd) affects the expression of inflammation-related genes in murine macrophages (RAW264 cells). DNA microarray analysis indicated that granulocyte colony-stimulating factor (G-CSF) showed the largest increase in expression in gAd-stimulated RAW264 cells. The gAd-induced secretion of G-CSF increased in a time- and dose-dependent manner. U0126 (MEK1/2 inhibitor) and PD98059 (MEK1 inhibitor) reduced the gAd-induced G-CSF mRNA expression and G-CSF protein production. gAd induced the phosphorylation of MEK1/2 and ERK1/2 in RAW264 cells. In addition, the gAd-induced phosphorylation of MEK1/2 and ERK1/2 was dramatically reduced by PD98059 and U0126, respectively. Collectively, these results suggest that MEK1/2-ERK1/2 signaling is involved in the adiponectin-induced secretion of G-CSF.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume292
Issue number1-2
DOIs
Publication statusPublished - Sep 24 2008

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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