Induction of light chain replacement in human plasma cells by caffeine is independent from both the upregulation of RAG protein expression and germ line transcription

Hirofumi Tachibana, Hirotaka Haruta, Kyoko Ueda, Takanori Chiwata, Koji Yamada

Research output: Contribution to journalArticle

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Abstract

When some human plasma cell lines are cultured with concanavalin A, the original light chain is replaced with another light chain which results from secondary VJ recombination (light chain shifting). We examined various intracellular factors involved in the induction of light chain shifting. Light chain shifting can be induced upon treatment with agents with phosphatase inhibitory activity such as caffeine and okadaic acid. Although the plasma cells used express both RAG-1 and RAG-2, the expression level of these proteins was not affected by caffeine or okadaic acid. Transcription of the germ line locus, which correlates to the locus activation for rearrangement, is also not influenced by phosphatase inhibition. However, the amount of signal broken-ended DNA intermediates generated during V(D)J rearrangement was shown to increase upon caffeine or okadaic acid treatment. The inhibitory activity of caffeine on phosphatase was the same as okadaic acid. However, caffeine exhibited much higher activity for VJ coding joint formation than okadaic acid. Therefore, although phosphatase inhibition might act, in part, on a mechanism by which V(D)J recombinase activity is regulated within the human plasma cells, other factor(s) are probably also involved in the process.

Original languageEnglish
Pages (from-to)5927-5933
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number8
DOIs
Publication statusPublished - Feb 25 2000

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Plasma (human)
Okadaic Acid
Transcription
Plasma Cells
Caffeine
Germ Cells
Up-Regulation
Phosphoric Monoester Hydrolases
Light
Proteins
VDJ Recombinases
Concanavalin A
Genetic Recombination
Joints
Chemical activation
Cells
Plasmas
Cell Line
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Induction of light chain replacement in human plasma cells by caffeine is independent from both the upregulation of RAG protein expression and germ line transcription. / Tachibana, Hirofumi; Haruta, Hirotaka; Ueda, Kyoko; Chiwata, Takanori; Yamada, Koji.

In: Journal of Biological Chemistry, Vol. 275, No. 8, 25.02.2000, p. 5927-5933.

Research output: Contribution to journalArticle

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