Induction of metallothionein in astrocytes by cytokines and heavy metals

Junichi Sawada, Yuíaka Kikuchi, Makoto Shibutani, Kunitoshi Mitsumori, Kazuhide Inoue, Tadashi Kasahara

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Immunohistochemical staining of human and rat brains for metallothionein (MT) using a monoclonal anti-MT antibody (MT45) revealed that protoplasmic astrocytes, which are densely localized in the gray matter, contain high levels of MT. Human U373MG astrocytoma cells were stimulated with interleukin (IL)-l or heavy metals to produce MT. When expression of MT in U373MG cells was analyzed by Northern blotting or indirect immunofluorescence using the MT45 antibody, it was found that IL-1 (≥ 10 U/ml), CdCl2 (50 μM) and high concentrations of ZnCL (500 μM) induced marked biosynthesis of MT. IL-6 (up to 3, 000 U/ml) and lower concentrations of ZnCl2 (10-50 μM), however, showed little inducing activity. Hippocampal astrocytes in primary culture produced a relatively high basal level of MT. The MT level increased in response to addition of IL-1 (≥ 10 U/ml), ZnCl2 (50 μM) and CdCL (5 μM). However, the increase induced by IL-6 (1, 000 U/ml) was not very marked. The in vivo induction of MT in the brain by cytokines is not fully understood. How- ever, our data and other indirect evidence suggest that IL-1 may be a potent stimulator of MT induction in astrocytes. Furthermore, the astrocytoma cell line, U373MG, is a suitable in vitro system to analyze the expression of MT in astrocytes.

Original languageEnglish
Pages (from-to)157-168
Number of pages12
JournalNeuroSignals
Volume3
Issue number3
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Fingerprint

Metallothionein
Heavy Metals
Astrocytes
Cytokines
Interleukin-1
Astrocytoma
Interleukin-10
Interleukin-6
Cadmium Chloride
Interleukins
Brain
Indirect Fluorescent Antibody Technique
Northern Blotting
Anti-Idiotypic Antibodies
Monoclonal Antibodies
Staining and Labeling
Cell Line

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Sawada, J., Kikuchi, Y., Shibutani, M., Mitsumori, K., Inoue, K., & Kasahara, T. (1994). Induction of metallothionein in astrocytes by cytokines and heavy metals. NeuroSignals, 3(3), 157-168. https://doi.org/10.1159/000109539

Induction of metallothionein in astrocytes by cytokines and heavy metals. / Sawada, Junichi; Kikuchi, Yuíaka; Shibutani, Makoto; Mitsumori, Kunitoshi; Inoue, Kazuhide; Kasahara, Tadashi.

In: NeuroSignals, Vol. 3, No. 3, 01.01.1994, p. 157-168.

Research output: Contribution to journalArticle

Sawada, J, Kikuchi, Y, Shibutani, M, Mitsumori, K, Inoue, K & Kasahara, T 1994, 'Induction of metallothionein in astrocytes by cytokines and heavy metals', NeuroSignals, vol. 3, no. 3, pp. 157-168. https://doi.org/10.1159/000109539
Sawada J, Kikuchi Y, Shibutani M, Mitsumori K, Inoue K, Kasahara T. Induction of metallothionein in astrocytes by cytokines and heavy metals. NeuroSignals. 1994 Jan 1;3(3):157-168. https://doi.org/10.1159/000109539
Sawada, Junichi ; Kikuchi, Yuíaka ; Shibutani, Makoto ; Mitsumori, Kunitoshi ; Inoue, Kazuhide ; Kasahara, Tadashi. / Induction of metallothionein in astrocytes by cytokines and heavy metals. In: NeuroSignals. 1994 ; Vol. 3, No. 3. pp. 157-168.
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