Induction of Mononuclear Precursor Cells with Osteoclastic Phenotypes in a Rat Bone Marrow Culture System Depleted of Stromal Cells

Akiko Kukita, Toshio Kukita, Jun Ho Shin, Osamu Kohashi

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Abstract

The importance of bone stromal cell involvement in osteoclast differentiation has been suggested. However, the detailed mechanism of its regulation is unclear. We investigated whether the soluble factors from osteoblastic cells influence osteoclast lineage cells in a population of rat bone marrow cells depleted of stromal cells. We show here that hematopoietic progenitor cells differentiate into mononuclear precursor cells, the preosteoclasts, that strongly express certain osteoclastic phenotypes in the absence of stromal cells. The multinucleation of the preosteoclasts occurred only in the presence of 1,25 dihydroxyvitamin D3 and stromal cells. The preosteoclasts themselves did not have dentine-resorbing activity, but they could differentiate into multinucleate osteoclast-like cells having such activity in the presence of rat primary osteoblasts. This culture system is a unique differentiation system for preosteoclast induction.

Original languageEnglish
Pages (from-to)1383-1389
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume196
Issue number3
DOIs
Publication statusPublished - Jan 1 1993

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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