Abstract
A study was made of the induction of phenol-sulfate conjugating activity by exposure to some phenolic compounds and also the duration of its activity induced by pentachlorophenol (PCP) in the mid-gut gland of short-necked clam Ruditapes philippinarum. In a preliminary experiment comparing the inductivity of phenol-sulfate conjugating activity in short-necked clam among PCP, resorcinol, p-cresol, p-chlorophenol, p-nitrophenol and phenol; PCP was the most effective inducer, resulting in a 2.4-fold activity in the control clam during the 3-day exposure period to 0.1 ppm PCP. Therefore, a subsequent experiment was performed to elucidate the effect of long-term exposure to sublethal PCP (0.1 ppm) for 5 weeks on the phenolsulfate conjugating activity in clams, feeding on a unicellular green alga Dunaliella sp. by dipping the clams in its growing sea water-medium (ca. 4 x 104 cells/m/) for 2–3 h every other day, when the test PCP-water was replaced by a newly prepared one. The conjugating activity in the clam increased with the time of exposure to PCP and reached 42 nmol/min/g-tissue at 5-week period, corresponding to ca. 7-fold with that in the control. When the clams which were exposed to 0.1 ppm PCP for 2 weeks and then transferred to PCP-free sea water, its induced activity of 24 nmol/ min/g-tissue decreased gradually, but it was still at a level of 1.5-fold with that in the control even after 3 weeks, although the biological half-life of PCP accumulated in the clam was only ca. 1 h.
| Original language | English |
|---|---|
| Pages (from-to) | 2073-2076 |
| Number of pages | 4 |
| Journal | Nippon Suisan Gakkaishi |
| Volume | 53 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Jan 1 1987 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Aquatic Science
Cite this
Induction of Phenol-Sulfate Conjugating Activity by Exposure to Phenols and Duration of Its Induced Activity in Short-necked Clam. / Kobayashi, Kunio; Oshima, Yuji; Hamada, Shigehito; Taguchi, Chie.
In: Nippon Suisan Gakkaishi, Vol. 53, No. 11, 01.01.1987, p. 2073-2076.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of Phenol-Sulfate Conjugating Activity by Exposure to Phenols and Duration of Its Induced Activity in Short-necked Clam
AU - Kobayashi, Kunio
AU - Oshima, Yuji
AU - Hamada, Shigehito
AU - Taguchi, Chie
PY - 1987/1/1
Y1 - 1987/1/1
N2 - A study was made of the induction of phenol-sulfate conjugating activity by exposure to some phenolic compounds and also the duration of its activity induced by pentachlorophenol (PCP) in the mid-gut gland of short-necked clam Ruditapes philippinarum. In a preliminary experiment comparing the inductivity of phenol-sulfate conjugating activity in short-necked clam among PCP, resorcinol, p-cresol, p-chlorophenol, p-nitrophenol and phenol; PCP was the most effective inducer, resulting in a 2.4-fold activity in the control clam during the 3-day exposure period to 0.1 ppm PCP. Therefore, a subsequent experiment was performed to elucidate the effect of long-term exposure to sublethal PCP (0.1 ppm) for 5 weeks on the phenolsulfate conjugating activity in clams, feeding on a unicellular green alga Dunaliella sp. by dipping the clams in its growing sea water-medium (ca. 4 x 104 cells/m/) for 2–3 h every other day, when the test PCP-water was replaced by a newly prepared one. The conjugating activity in the clam increased with the time of exposure to PCP and reached 42 nmol/min/g-tissue at 5-week period, corresponding to ca. 7-fold with that in the control. When the clams which were exposed to 0.1 ppm PCP for 2 weeks and then transferred to PCP-free sea water, its induced activity of 24 nmol/ min/g-tissue decreased gradually, but it was still at a level of 1.5-fold with that in the control even after 3 weeks, although the biological half-life of PCP accumulated in the clam was only ca. 1 h.
AB - A study was made of the induction of phenol-sulfate conjugating activity by exposure to some phenolic compounds and also the duration of its activity induced by pentachlorophenol (PCP) in the mid-gut gland of short-necked clam Ruditapes philippinarum. In a preliminary experiment comparing the inductivity of phenol-sulfate conjugating activity in short-necked clam among PCP, resorcinol, p-cresol, p-chlorophenol, p-nitrophenol and phenol; PCP was the most effective inducer, resulting in a 2.4-fold activity in the control clam during the 3-day exposure period to 0.1 ppm PCP. Therefore, a subsequent experiment was performed to elucidate the effect of long-term exposure to sublethal PCP (0.1 ppm) for 5 weeks on the phenolsulfate conjugating activity in clams, feeding on a unicellular green alga Dunaliella sp. by dipping the clams in its growing sea water-medium (ca. 4 x 104 cells/m/) for 2–3 h every other day, when the test PCP-water was replaced by a newly prepared one. The conjugating activity in the clam increased with the time of exposure to PCP and reached 42 nmol/min/g-tissue at 5-week period, corresponding to ca. 7-fold with that in the control. When the clams which were exposed to 0.1 ppm PCP for 2 weeks and then transferred to PCP-free sea water, its induced activity of 24 nmol/ min/g-tissue decreased gradually, but it was still at a level of 1.5-fold with that in the control even after 3 weeks, although the biological half-life of PCP accumulated in the clam was only ca. 1 h.
UR - http://www.scopus.com/inward/record.url?scp=85008068649&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008068649&partnerID=8YFLogxK
U2 - 10.2331/suisan.53.2073
DO - 10.2331/suisan.53.2073
M3 - Article
AN - SCOPUS:85008068649
VL - 53
SP - 2073
EP - 2076
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 11
ER -