Induction of platelet-derived growth factor β-receptor in focal ischemia of rat brain

Koji Iihara, Masakiyo Sasahara, Nobuo Hashimoto, Fumitada Hazama

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Our previous study on the ischemia-induced expression of platelet- derived growth factor (PDGF)-B chain in the rat brain prompted us to examine expression of PDGF β-receptor in the ischemic brain. Focal ischemia was induced by permanent tandem occlusion of middle cerebral and common carotid arteries in spontaneously hypertensive rats. Northern analysis revealed that ischemia significantly increased expression of the receptor in the ischemic neocortex at 4 and 7 days (328 ± 109%; 323 ± 119%, respectively, over control: n = 4, p < 0.05 versus sham). Neurons in infarct transiently showed increased immunostaining for the receptor at 1 day, whereas neurons in periinfarct area showed sustained and increased immunoreactivity from 1 to 14 days post-ischemia. Reactive glial cells in the external capsule and in molecular layer of the neocortex adjacent to infarct possessed enhanced immunoreactivity from 1 to 21 days. Furthermore, marked immunoreactivity was observed on brain macrophages in infarct and on the abluminal side of capillaries surrounding infarct from 4 to 7 days. These results demonstrated that ischemic insult increases expression of the PDGF β-receptor at both the mRNA and protein lever in the brain, suggesting its important role in cellular cascade of the ischemic brain.

Original languageEnglish
Pages (from-to)941-949
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume16
Issue number5
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Platelet-Derived Growth Factor Receptors
Brain Ischemia
Ischemia
Brain
Neocortex
Proto-Oncogene Proteins c-sis
Neurons
Common Carotid Artery
Inbred SHR Rats
Neuroglia
Macrophages
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Induction of platelet-derived growth factor β-receptor in focal ischemia of rat brain. / Iihara, Koji; Sasahara, Masakiyo; Hashimoto, Nobuo; Hazama, Fumitada.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 16, No. 5, 01.01.1996, p. 941-949.

Research output: Contribution to journalArticle

Iihara, Koji ; Sasahara, Masakiyo ; Hashimoto, Nobuo ; Hazama, Fumitada. / Induction of platelet-derived growth factor β-receptor in focal ischemia of rat brain. In: Journal of Cerebral Blood Flow and Metabolism. 1996 ; Vol. 16, No. 5. pp. 941-949.
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