Induction of Salivary Gland-Like Cells from Dental Follicle Epithelial Cells

Q. L. Xu, Akihiro Furuhashi, Q. Z. Zhang, C. M. Jiang, T. H. Chang, A. D. Le

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The dental follicle (DF), most often associated with unerupted teeth, is a condensation of ectomesenchymal cells that surrounds the tooth germ in early stages of tooth development. In the present study, we aim to isolate epithelial stem-like cells from the human DF and explore their potential differentiation into salivary gland (SG) cells. We demonstrated the expression of stem cell-related genes in the epithelial components of human DF tissues, and these epithelial progenitor cells could be isolated and ex vivo expanded in a reproducible manner. The human DF-derived epithelial cells possessed clonogenic and sphere-forming capabilities, as well as expressed a panel of epithelial stem cell-related genes, thus conferring stem cell properties (hDF-EpiSCs). When cultured under in vitro 3-dimensional induction conditions, hDF-EpiSCs were capable to differentiate into SG acinar and duct cells. Furthermore, transplantation of hDF-EpiSC-loaded native de-cellularized rat parotid gland scaffolds into the renal capsule of nude mice led to the differentiation of transplanted hDF-EpiSCs into salivary gland-like cells. These findings suggest that hDF-EpiSCs might be a promising source of epithelial stem cells for the development of stem cell-based therapy or bioengineering SG tissues to repair/regenerate SG dysfunction.

Original languageEnglish
Pages (from-to)1035-1043
Number of pages9
JournalJournal of Dental Research
Volume96
Issue number9
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Dental Sac
Salivary Glands
Stem Cells
Epithelial Cells
Unerupted Tooth
Tooth Germ
Salivary Ducts
Bioengineering
Acinar Cells
Parotid Gland
Cell- and Tissue-Based Therapy
Nude Mice
Genes
Capsules
Tooth
Transplantation
Kidney

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Induction of Salivary Gland-Like Cells from Dental Follicle Epithelial Cells. / Xu, Q. L.; Furuhashi, Akihiro; Zhang, Q. Z.; Jiang, C. M.; Chang, T. H.; Le, A. D.

In: Journal of Dental Research, Vol. 96, No. 9, 01.08.2017, p. 1035-1043.

Research output: Contribution to journalArticle

Xu, Q. L. ; Furuhashi, Akihiro ; Zhang, Q. Z. ; Jiang, C. M. ; Chang, T. H. ; Le, A. D. / Induction of Salivary Gland-Like Cells from Dental Follicle Epithelial Cells. In: Journal of Dental Research. 2017 ; Vol. 96, No. 9. pp. 1035-1043.
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