Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid

Yuji Tokumoto, Hiroyuki Kajiura, Shinya Takeno, Yoko Harada, Nobuaki Suzuki, Takenori Hosaka, Koichiro Gyokusen, Yoshihisa Nakazawa

Research output: Contribution to journalComment/debate

5 Citations (Scopus)

Abstract

Polyploid plants tend to have larger organs than diploids due to the doubling of their chromosome number. However, the enlarged regions, whether cells, tissues, or individuals differ depending on the plant species or taxon, as have metabolic changes. Eucommia ulmoides is a deciduous dioecious plant natively distributed in China that accumulates trans- 1,4-polyisoprene (TPI) in laticifers throughout its tissues. To induce tetraploids of E. ulmoides, colchicine treatment time and concentration were assessed for ability to obtain tetraploids, and the resulting plants were analyzed for phenotypic differences from diploids with respect to individual size, 16 leaf attributes, sizes of three cell types (epidermis, laticifer, and parenchyma), and TPI content and molecular weight. Effective conditions for obtaining tetraploids were 0.05% colchicine for 48 h, which led to obtaining a total of 9 tetraploids. The tetraploids showed larger stomata size but lower stomata density than diploids. Significant differences in the mean values of other attributes including leaf size, cell size, and TPI content of tetraploids could not be detected between tetraploids and diploids, suggesting that E. ulmoides is less affected by gene doubling than other plant species, and that tetraploid individuals might not have a tendency to increase their biomass.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalPlant Biotechnology
Volume33
Issue number1
DOIs
Publication statusPublished - Apr 22 2016

Fingerprint

Eucommiaceae
Hevea
Eucommia ulmoides
Tetraploidy
Hevea brasiliensis
Diploidy
tetraploidy
diploidy
colchicine
Colchicine
Cell Size
stomata
Polyploidy
cells
epidermis (plant)
Epidermis
polyploidy
Biomass
parenchyma (plant tissue)
chromosome number

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Tokumoto, Y., Kajiura, H., Takeno, S., Harada, Y., Suzuki, N., Hosaka, T., ... Nakazawa, Y. (2016). Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid. Plant Biotechnology, 33(1), 51-57. https://doi.org/10.5511/plantbiotechnology.15.1219a

Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid. / Tokumoto, Yuji; Kajiura, Hiroyuki; Takeno, Shinya; Harada, Yoko; Suzuki, Nobuaki; Hosaka, Takenori; Gyokusen, Koichiro; Nakazawa, Yoshihisa.

In: Plant Biotechnology, Vol. 33, No. 1, 22.04.2016, p. 51-57.

Research output: Contribution to journalComment/debate

Tokumoto, Y, Kajiura, H, Takeno, S, Harada, Y, Suzuki, N, Hosaka, T, Gyokusen, K & Nakazawa, Y 2016, 'Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid', Plant Biotechnology, vol. 33, no. 1, pp. 51-57. https://doi.org/10.5511/plantbiotechnology.15.1219a
Tokumoto, Yuji ; Kajiura, Hiroyuki ; Takeno, Shinya ; Harada, Yoko ; Suzuki, Nobuaki ; Hosaka, Takenori ; Gyokusen, Koichiro ; Nakazawa, Yoshihisa. / Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid. In: Plant Biotechnology. 2016 ; Vol. 33, No. 1. pp. 51-57.
@article{db43d26e0153407e98d660c5f0fc2565,
title = "Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid",
abstract = "Polyploid plants tend to have larger organs than diploids due to the doubling of their chromosome number. However, the enlarged regions, whether cells, tissues, or individuals differ depending on the plant species or taxon, as have metabolic changes. Eucommia ulmoides is a deciduous dioecious plant natively distributed in China that accumulates trans- 1,4-polyisoprene (TPI) in laticifers throughout its tissues. To induce tetraploids of E. ulmoides, colchicine treatment time and concentration were assessed for ability to obtain tetraploids, and the resulting plants were analyzed for phenotypic differences from diploids with respect to individual size, 16 leaf attributes, sizes of three cell types (epidermis, laticifer, and parenchyma), and TPI content and molecular weight. Effective conditions for obtaining tetraploids were 0.05{\%} colchicine for 48 h, which led to obtaining a total of 9 tetraploids. The tetraploids showed larger stomata size but lower stomata density than diploids. Significant differences in the mean values of other attributes including leaf size, cell size, and TPI content of tetraploids could not be detected between tetraploids and diploids, suggesting that E. ulmoides is less affected by gene doubling than other plant species, and that tetraploid individuals might not have a tendency to increase their biomass.",
author = "Yuji Tokumoto and Hiroyuki Kajiura and Shinya Takeno and Yoko Harada and Nobuaki Suzuki and Takenori Hosaka and Koichiro Gyokusen and Yoshihisa Nakazawa",
year = "2016",
month = "4",
day = "22",
doi = "10.5511/plantbiotechnology.15.1219a",
language = "English",
volume = "33",
pages = "51--57",
journal = "Plant Biotechnology",
issn = "1342-4580",
publisher = "Japanese Society for Plant Cell and Molecular Biology",
number = "1",

}

TY - JOUR

T1 - Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid

AU - Tokumoto, Yuji

AU - Kajiura, Hiroyuki

AU - Takeno, Shinya

AU - Harada, Yoko

AU - Suzuki, Nobuaki

AU - Hosaka, Takenori

AU - Gyokusen, Koichiro

AU - Nakazawa, Yoshihisa

PY - 2016/4/22

Y1 - 2016/4/22

N2 - Polyploid plants tend to have larger organs than diploids due to the doubling of their chromosome number. However, the enlarged regions, whether cells, tissues, or individuals differ depending on the plant species or taxon, as have metabolic changes. Eucommia ulmoides is a deciduous dioecious plant natively distributed in China that accumulates trans- 1,4-polyisoprene (TPI) in laticifers throughout its tissues. To induce tetraploids of E. ulmoides, colchicine treatment time and concentration were assessed for ability to obtain tetraploids, and the resulting plants were analyzed for phenotypic differences from diploids with respect to individual size, 16 leaf attributes, sizes of three cell types (epidermis, laticifer, and parenchyma), and TPI content and molecular weight. Effective conditions for obtaining tetraploids were 0.05% colchicine for 48 h, which led to obtaining a total of 9 tetraploids. The tetraploids showed larger stomata size but lower stomata density than diploids. Significant differences in the mean values of other attributes including leaf size, cell size, and TPI content of tetraploids could not be detected between tetraploids and diploids, suggesting that E. ulmoides is less affected by gene doubling than other plant species, and that tetraploid individuals might not have a tendency to increase their biomass.

AB - Polyploid plants tend to have larger organs than diploids due to the doubling of their chromosome number. However, the enlarged regions, whether cells, tissues, or individuals differ depending on the plant species or taxon, as have metabolic changes. Eucommia ulmoides is a deciduous dioecious plant natively distributed in China that accumulates trans- 1,4-polyisoprene (TPI) in laticifers throughout its tissues. To induce tetraploids of E. ulmoides, colchicine treatment time and concentration were assessed for ability to obtain tetraploids, and the resulting plants were analyzed for phenotypic differences from diploids with respect to individual size, 16 leaf attributes, sizes of three cell types (epidermis, laticifer, and parenchyma), and TPI content and molecular weight. Effective conditions for obtaining tetraploids were 0.05% colchicine for 48 h, which led to obtaining a total of 9 tetraploids. The tetraploids showed larger stomata size but lower stomata density than diploids. Significant differences in the mean values of other attributes including leaf size, cell size, and TPI content of tetraploids could not be detected between tetraploids and diploids, suggesting that E. ulmoides is less affected by gene doubling than other plant species, and that tetraploid individuals might not have a tendency to increase their biomass.

UR - http://www.scopus.com/inward/record.url?scp=84964304836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964304836&partnerID=8YFLogxK

U2 - 10.5511/plantbiotechnology.15.1219a

DO - 10.5511/plantbiotechnology.15.1219a

M3 - Comment/debate

AN - SCOPUS:84964304836

VL - 33

SP - 51

EP - 57

JO - Plant Biotechnology

JF - Plant Biotechnology

SN - 1342-4580

IS - 1

ER -