Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments

Nguyen Thi Phuong Thao, Kenji Ureshino, Ikuo Miyajima, Yukio Ozaki, Hiroshi Okubo

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Tetraploids were induced successfully from in vitro plants of diploid Alocasia 'Green Velvet' (2n=28) by treating shoot tips with colchicine or oryzalin. Colchicine at a concentration of 0.01, 0.05, 0.1% and oryzalin at 0.005, 0.01, 0.05% (both were dissolved in 1% DMSO, dimethylsulfoxide) were tested for 24, 48 and 72 h. Ploidy levels could be easily determined by flow cytometry. In total, 396 plantlets were investigated. Controls with DMSO maintained the diploid status; 22 tetraploids and 22 chimeras were obtained from treatments with colchicine and oryzalin. The most efficient condition for inducing tetraploids seemed to be treatment with 0.01% oryzalin for 24 h. Variation in the morphological characteristic of leaf shape was found among the multiplied plants under the same growing condition. While the leaves of diploids had elongated-heart shape, the leaves of tetraploids and chimeras tended to be round.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume72
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

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Alocasia
oryzalin
colchicine
tetraploidy
diploidy
chimerism
leaves
dimethyl sulfoxide
ploidy
flow cytometry
plantlets
heart

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. / Thao, Nguyen Thi Phuong; Ureshino, Kenji; Miyajima, Ikuo; Ozaki, Yukio; Okubo, Hiroshi.

In: Plant Cell, Tissue and Organ Culture, Vol. 72, No. 1, 01.01.2003, p. 19-25.

Research output: Contribution to journalArticle

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