Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27

Mohamed Nassef; Takahiko Tawaratsumita; Yumi Oba; Hina Satone; Kei Nakayama; Yohei Shimasaki; Tsuneo Honjo; Yuji Oshima

doi:10.1016/j.marpolbul.2010.12.005

Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27

Mohamed Nassef, Takahiko Tawaratsumita, Yumi Oba, Hina Satone, Kei Nakayama, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima

Animal and Marine Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In this study, individual Japanese flounder were intraperitoneally injected with 2μg tributyltin-d27 (TBT-d27) fish^-1. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology.

Original language	English
Pages (from-to)	412-414
Number of pages	3
Journal	Marine Pollution Bulletin
Volume	62
Issue number	2
DOIs	https://doi.org/10.1016/j.marpolbul.2010.12.005
Publication status	Published - Feb 2011

All Science Journal Classification (ASJC) codes

Oceanography
Aquatic Science
Pollution

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10.1016/j.marpolbul.2010.12.005

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title = "Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27",

abstract = "In this study, individual Japanese flounder were intraperitoneally injected with 2μg tributyltin-d27 (TBT-d27) fish-1. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology.",

author = "Mohamed Nassef and Takahiko Tawaratsumita and Yumi Oba and Hina Satone and Kei Nakayama and Yohei Shimasaki and Tsuneo Honjo and Yuji Oshima",

year = "2011",

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doi = "10.1016/j.marpolbul.2010.12.005",

language = "English",

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journal = "Marine Pollution Bulletin",

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T1 - Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27

AU - Nassef, Mohamed

AU - Tawaratsumita, Takahiko

AU - Oba, Yumi

AU - Satone, Hina

AU - Nakayama, Kei

AU - Shimasaki, Yohei

AU - Honjo, Tsuneo

AU - Oshima, Yuji

PY - 2011/2

Y1 - 2011/2

N2 - In this study, individual Japanese flounder were intraperitoneally injected with 2μg tributyltin-d27 (TBT-d27) fish-1. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology.

AB - In this study, individual Japanese flounder were intraperitoneally injected with 2μg tributyltin-d27 (TBT-d27) fish-1. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology.

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Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27

Abstract

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