Infection and development of vairimorpha sp. NIS M12(microsporida: Protozoa) in a lepidopteran cell line

Shino Inoue, Chisa Yasunaga-Aoki, Masako Funakoshi, Takeshi Kawarabata, Syoji Hayasaka

Research output: Contribution to journalArticle

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Abstract

Vairimorpha sp. NIS M12, a microsporidian parasite isolated from the silkworm, Bombyx mori, successfully infected the cell line of Spodoptera frugiperda SF21AE II. After priming with 0.1N KOH solution, the spores were mixed thoroughly with S. frugiperda cells suspended in Rinaldini's solution. Inoculated cells were cultured at 28°C in IPL-41 medium supplemented with 10% fetal bovine serum. At a ratio of 30 spores per cell, 9.1% of S. frugiperda cells were infected initially with sporoplasms 1hr postinoculation. About 65% of the infected cells harbored a single sporoplasm. The spread of infection and spore production of V. sp. NIS M12 in cell cultures were markedly different from those of Nosema bombycis, while the in vitro life cycles of the two parasites was similar. Short-coiled spores occurred 72hr postinoculation, and longcoiled mature spores were produced 10 days postinoculation. No formation of syncytia was observed in Vairimorpha infected cell cultures.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalJournal of Sericultural Science of Japan
Volume64
Issue number1
DOIs
Publication statusPublished - Jan 1 1995

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Microsporida
Vairimorpha
Protozoa
Microsporidia
Spores
Cell culture
spores
Spodoptera
Lepidoptera
Cells
cell lines
Spodoptera frugiperda
Cell Line
Infection
Bombyx
infection
Life cycle
cells
Parasites
cell culture

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

Cite this

Infection and development of vairimorpha sp. NIS M12(microsporida : Protozoa) in a lepidopteran cell line. / Inoue, Shino; Yasunaga-Aoki, Chisa; Funakoshi, Masako; Kawarabata, Takeshi; Hayasaka, Syoji.

In: Journal of Sericultural Science of Japan, Vol. 64, No. 1, 01.01.1995, p. 39-45.

Research output: Contribution to journalArticle

Inoue, Shino ; Yasunaga-Aoki, Chisa ; Funakoshi, Masako ; Kawarabata, Takeshi ; Hayasaka, Syoji. / Infection and development of vairimorpha sp. NIS M12(microsporida : Protozoa) in a lepidopteran cell line. In: Journal of Sericultural Science of Japan. 1995 ; Vol. 64, No. 1. pp. 39-45.
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