Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus

Minoru Takeshita, Emiko Koizumi, Makiko Noguchi, Kae Sueda, Hanako Shimura, Noriko Ishikawa, Hideyuki Matsuura, Kazusato Ohshima, Tomohide Natsuaki, Shigeru Kuwata, Naruto Furuya, Kenichi Tsuchiya, Chikara Masuta

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Turnip mosaic virus (TuMV) induced more severe symptoms on Nicotiana benthamiana than single infection. To dissect the relationships between spatial infection patterns and the 2b protein (2b) of CMV in single or mixed infections, the CMV vectors expressing enhanced green fluorescent or Discosoma sp. red fluorescent proteins (EGFP [EG] or DsRed2 [Ds], respectively) were constructed from the same wild-type CMVY and used for inoculation onto N. benthamiana. CMV2-A1 vector (C2-A1 [A1]) has a functional 2b while CMV-H1 vector (C2-H1 [H1]) is 2b deficient. As we expected from the 2b function as an RNA silencing suppressor (RSS), in a single infection, A1Ds retained a high level of accumulation at initial infection sites and showed extensive fluorescence in upper, noninoculated leaves, whereas H1Ds disappeared rapidly at initial infection sites and could not spread efficiently in upper, noninoculated leaf tissues. In various mixed infections, we found two phenomena providing novel insights into the relationships among RSS, viral synergism, and interference. First, H1Ds could not spread efficiently from vasculature into nonvascular tissues with or without TuMV, suggesting that RNA silencing was not involved in CMV unloading from vasculature. These results indicated that 2b could promote CMV to unload from vasculature into nonvascular tissues, and that this 2b function might be independent of its RSS activity. Second, we detected spatial interference (local interference) between A1Ds and A1EG in mixed infection with TuMV, between A1Ds (or H1Ds) and TuMV, and between H1Ds and H1EG. This observation suggested that local interference between two viruses was established even in the synergism between CMV and TuMV and, again, RNA silencing did not seem to contribute greatly to this phenomenon.

Original languageEnglish
Pages (from-to)18-27
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 1 2012

Fingerprint

Viral Interference
Cucumovirus
Turnip mosaic virus
Mosaic Viruses
Brassica napus
Cucumber mosaic virus
synergism
RNA Interference
RNA interference
Coinfection
mixed infection
Infection
infection
Nicotiana benthamiana
signs and symptoms (plants)
Tobacco
leaves
Fluorescence
fluorescence
Viruses

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus. / Takeshita, Minoru; Koizumi, Emiko; Noguchi, Makiko; Sueda, Kae; Shimura, Hanako; Ishikawa, Noriko; Matsuura, Hideyuki; Ohshima, Kazusato; Natsuaki, Tomohide; Kuwata, Shigeru; Furuya, Naruto; Tsuchiya, Kenichi; Masuta, Chikara.

In: Molecular Plant-Microbe Interactions, Vol. 25, No. 1, 01.01.2012, p. 18-27.

Research output: Contribution to journalArticle

Takeshita, M, Koizumi, E, Noguchi, M, Sueda, K, Shimura, H, Ishikawa, N, Matsuura, H, Ohshima, K, Natsuaki, T, Kuwata, S, Furuya, N, Tsuchiya, K & Masuta, C 2012, 'Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus', Molecular Plant-Microbe Interactions, vol. 25, no. 1, pp. 18-27. https://doi.org/10.1094/MPMI-06-11-0170
Takeshita, Minoru ; Koizumi, Emiko ; Noguchi, Makiko ; Sueda, Kae ; Shimura, Hanako ; Ishikawa, Noriko ; Matsuura, Hideyuki ; Ohshima, Kazusato ; Natsuaki, Tomohide ; Kuwata, Shigeru ; Furuya, Naruto ; Tsuchiya, Kenichi ; Masuta, Chikara. / Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus. In: Molecular Plant-Microbe Interactions. 2012 ; Vol. 25, No. 1. pp. 18-27.
@article{7e91ce1f0b4b46059831a063d34347c6,
title = "Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus",
abstract = "Turnip mosaic virus (TuMV) induced more severe symptoms on Nicotiana benthamiana than single infection. To dissect the relationships between spatial infection patterns and the 2b protein (2b) of CMV in single or mixed infections, the CMV vectors expressing enhanced green fluorescent or Discosoma sp. red fluorescent proteins (EGFP [EG] or DsRed2 [Ds], respectively) were constructed from the same wild-type CMVY and used for inoculation onto N. benthamiana. CMV2-A1 vector (C2-A1 [A1]) has a functional 2b while CMV-H1 vector (C2-H1 [H1]) is 2b deficient. As we expected from the 2b function as an RNA silencing suppressor (RSS), in a single infection, A1Ds retained a high level of accumulation at initial infection sites and showed extensive fluorescence in upper, noninoculated leaves, whereas H1Ds disappeared rapidly at initial infection sites and could not spread efficiently in upper, noninoculated leaf tissues. In various mixed infections, we found two phenomena providing novel insights into the relationships among RSS, viral synergism, and interference. First, H1Ds could not spread efficiently from vasculature into nonvascular tissues with or without TuMV, suggesting that RNA silencing was not involved in CMV unloading from vasculature. These results indicated that 2b could promote CMV to unload from vasculature into nonvascular tissues, and that this 2b function might be independent of its RSS activity. Second, we detected spatial interference (local interference) between A1Ds and A1EG in mixed infection with TuMV, between A1Ds (or H1Ds) and TuMV, and between H1Ds and H1EG. This observation suggested that local interference between two viruses was established even in the synergism between CMV and TuMV and, again, RNA silencing did not seem to contribute greatly to this phenomenon.",
author = "Minoru Takeshita and Emiko Koizumi and Makiko Noguchi and Kae Sueda and Hanako Shimura and Noriko Ishikawa and Hideyuki Matsuura and Kazusato Ohshima and Tomohide Natsuaki and Shigeru Kuwata and Naruto Furuya and Kenichi Tsuchiya and Chikara Masuta",
year = "2012",
month = "1",
day = "1",
doi = "10.1094/MPMI-06-11-0170",
language = "English",
volume = "25",
pages = "18--27",
journal = "Molecular Plant-Microbe Interactions",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "1",

}

TY - JOUR

T1 - Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus

AU - Takeshita, Minoru

AU - Koizumi, Emiko

AU - Noguchi, Makiko

AU - Sueda, Kae

AU - Shimura, Hanako

AU - Ishikawa, Noriko

AU - Matsuura, Hideyuki

AU - Ohshima, Kazusato

AU - Natsuaki, Tomohide

AU - Kuwata, Shigeru

AU - Furuya, Naruto

AU - Tsuchiya, Kenichi

AU - Masuta, Chikara

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Turnip mosaic virus (TuMV) induced more severe symptoms on Nicotiana benthamiana than single infection. To dissect the relationships between spatial infection patterns and the 2b protein (2b) of CMV in single or mixed infections, the CMV vectors expressing enhanced green fluorescent or Discosoma sp. red fluorescent proteins (EGFP [EG] or DsRed2 [Ds], respectively) were constructed from the same wild-type CMVY and used for inoculation onto N. benthamiana. CMV2-A1 vector (C2-A1 [A1]) has a functional 2b while CMV-H1 vector (C2-H1 [H1]) is 2b deficient. As we expected from the 2b function as an RNA silencing suppressor (RSS), in a single infection, A1Ds retained a high level of accumulation at initial infection sites and showed extensive fluorescence in upper, noninoculated leaves, whereas H1Ds disappeared rapidly at initial infection sites and could not spread efficiently in upper, noninoculated leaf tissues. In various mixed infections, we found two phenomena providing novel insights into the relationships among RSS, viral synergism, and interference. First, H1Ds could not spread efficiently from vasculature into nonvascular tissues with or without TuMV, suggesting that RNA silencing was not involved in CMV unloading from vasculature. These results indicated that 2b could promote CMV to unload from vasculature into nonvascular tissues, and that this 2b function might be independent of its RSS activity. Second, we detected spatial interference (local interference) between A1Ds and A1EG in mixed infection with TuMV, between A1Ds (or H1Ds) and TuMV, and between H1Ds and H1EG. This observation suggested that local interference between two viruses was established even in the synergism between CMV and TuMV and, again, RNA silencing did not seem to contribute greatly to this phenomenon.

AB - Turnip mosaic virus (TuMV) induced more severe symptoms on Nicotiana benthamiana than single infection. To dissect the relationships between spatial infection patterns and the 2b protein (2b) of CMV in single or mixed infections, the CMV vectors expressing enhanced green fluorescent or Discosoma sp. red fluorescent proteins (EGFP [EG] or DsRed2 [Ds], respectively) were constructed from the same wild-type CMVY and used for inoculation onto N. benthamiana. CMV2-A1 vector (C2-A1 [A1]) has a functional 2b while CMV-H1 vector (C2-H1 [H1]) is 2b deficient. As we expected from the 2b function as an RNA silencing suppressor (RSS), in a single infection, A1Ds retained a high level of accumulation at initial infection sites and showed extensive fluorescence in upper, noninoculated leaves, whereas H1Ds disappeared rapidly at initial infection sites and could not spread efficiently in upper, noninoculated leaf tissues. In various mixed infections, we found two phenomena providing novel insights into the relationships among RSS, viral synergism, and interference. First, H1Ds could not spread efficiently from vasculature into nonvascular tissues with or without TuMV, suggesting that RNA silencing was not involved in CMV unloading from vasculature. These results indicated that 2b could promote CMV to unload from vasculature into nonvascular tissues, and that this 2b function might be independent of its RSS activity. Second, we detected spatial interference (local interference) between A1Ds and A1EG in mixed infection with TuMV, between A1Ds (or H1Ds) and TuMV, and between H1Ds and H1EG. This observation suggested that local interference between two viruses was established even in the synergism between CMV and TuMV and, again, RNA silencing did not seem to contribute greatly to this phenomenon.

UR - http://www.scopus.com/inward/record.url?scp=84055199870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84055199870&partnerID=8YFLogxK

U2 - 10.1094/MPMI-06-11-0170

DO - 10.1094/MPMI-06-11-0170

M3 - Article

C2 - 21916556

AN - SCOPUS:84055199870

VL - 25

SP - 18

EP - 27

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

SN - 0894-0282

IS - 1

ER -