Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus

Hiroshi Ishida, Minoru Ayata, Masashi Shingai, Isamu Matsunaga, Yoshiyuki Seto, Yuko Katayama, Nobuhiro Iritani, Tsukasa Seya, Yusuke Yanagi, Osamu Matsuoka, Tsunekazu Yamano, Hisashi Ogura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Measles virus is the causative agent of subacute sclerosing panencephalitis (SSPE). The viruses isolated from brain cells of patients with SSPE (called SSPE viruses) are defective in cell-free virus production in vitro. To investigate the cell tropism of three strains of SSPE virus (Osaka-1, Osaka-2, Osaka-3), SSPE virus-infected cell cultures were treated with cytochalasin D to prepare virus-like particles (CD-VLPs). All CD-VLPs formed syncytia after infection in CHO cells expressing CD150 but not in those expressing CD46. In addition, an antibody to CD46 did not block the infection of Vero cells by SSPE CD-VLPs. The results were consistent with our previous suggestion that one or more unidentified receptors might be involved in the entry process. Infection with the CD-VLPs from three SSPE strains was further examined in different human cell lines, including those of neural origin, and was found to induce syncytia in epithelial cells (HeLa and 293T) as well as neuroblastoma cells (IMR-32 and SK-N-SH) with varying efficiency. SSPE CD-VLPs also infected glioblastoma cells (A172) and astrocytoma cells (U-251) but syncytial formation was rarely induced. These epithelial and neural cell lines were not permissive for the replication of wild-type MV. Together with our previous observations, these results suggest that the cell entry receptor is the major factor determining the cell tropism of SSPE viruses. Further studies are necessary to identify other viral and/or cellular factors that might be involved in the replication of SSPE virus in specific neural cells and in the brain.

Original languageEnglish
Pages (from-to)277-287
Number of pages11
JournalMICROBIOLOGY and IMMUNOLOGY
Volume48
Issue number4
DOIs
Publication statusPublished - Jan 1 2004

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Subacute Sclerosing Panencephalitis
Viruses
Cytochalasin D
Cell Line
Infection
Virion
Tropism
Giant Cells
Epithelial Cells
Defective Viruses
Measles virus
Vero Cells
CHO Cells
Astrocytoma
Brain
Glioblastoma
Neuroblastoma
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus. / Ishida, Hiroshi; Ayata, Minoru; Shingai, Masashi; Matsunaga, Isamu; Seto, Yoshiyuki; Katayama, Yuko; Iritani, Nobuhiro; Seya, Tsukasa; Yanagi, Yusuke; Matsuoka, Osamu; Yamano, Tsunekazu; Ogura, Hisashi.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 48, No. 4, 01.01.2004, p. 277-287.

Research output: Contribution to journalArticle

Ishida, H, Ayata, M, Shingai, M, Matsunaga, I, Seto, Y, Katayama, Y, Iritani, N, Seya, T, Yanagi, Y, Matsuoka, O, Yamano, T & Ogura, H 2004, 'Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus', MICROBIOLOGY and IMMUNOLOGY, vol. 48, no. 4, pp. 277-287. https://doi.org/10.1111/j.1348-0421.2004.tb03524.x
Ishida, Hiroshi ; Ayata, Minoru ; Shingai, Masashi ; Matsunaga, Isamu ; Seto, Yoshiyuki ; Katayama, Yuko ; Iritani, Nobuhiro ; Seya, Tsukasa ; Yanagi, Yusuke ; Matsuoka, Osamu ; Yamano, Tsunekazu ; Ogura, Hisashi. / Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus. In: MICROBIOLOGY and IMMUNOLOGY. 2004 ; Vol. 48, No. 4. pp. 277-287.
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